Whether is genetic disturbance caused in coastal mraine species?: Genome-wide population genetics of the yellowfin goby

• Project/Area Number: 26850131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: The University of Tokyo
• Principal Investigator: Hirase Shotaro 東京大学, 大学院農学生命科学研究科(農学部), 助教 (90635559)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: マハゼ / 分子系統地理 / 遺伝的撹乱 / RAD-seq / 次世代シーケンサー / 侵入種 / 集団構造 / クラスタリング分析 / RADシーケンス / ボトルネック / ミトコンドリアDNA

Outline of Final Research Achievements

I performed mitochondrial DNA and RAD-seq analyses to investigate the population structure of Acanthogobius flavimanus which is expected to be transported by ballast water. Mitochondrial DNA analysis showed significant genetic differentiation among populations in the Japanese archipelago. This analysis also suggested that the origin of a northern California population is Tokyo Bay, but I was unable to identify the origin of a southern California population and a Melbourne population. RAD-seq analysis showed genetic differentiation between the Pacific Ocean and the Sea of Japan populations. The genetic structure related to geographic locations suggests that genetic disturbance has not been caused by ballast water in Acanthogobius flavimanus populations in the Japanese archipelago.

Research Products

• [Int'l Joint Research] CAPIM(Australia)
• [Int'l Joint Research] CAPIM of Melbourne University(Australia)
• [Journal Article] Phylogeography of the yellowfin goby Acanthogobius flavimanus in native and non-native distributions (2017)
  • Author(s): Hirase S, Chambers S, Hassell K, Carew M, Pettigrove V, Soyano K, Nagae M, Iwasaki W.
  • Journal Title: Marine Biology Volume: -
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Stability of genetic diversity in an intertidal goby against tsunami disturbances (2016)
  • Author(s): Hirase S, Ikeda M, Hayasaka S, Iwasaki W, Kijima A.
  • Journal Title: Marine Ecology Volume: 37 Issue: 5 Pages: 1161-1167
  • DOI: 10.1111/maec.12377
  • Peer Reviewed
• [Journal Article] Mitogenome Sequencing Alleviates Random Rooting Effect in Phylogeography. (2016)
  • Author(s): Shotaro Hirase, Hirohiko Takeshima, Mutsumi Nishida, Wataru Iwasaki
  • Journal Title: Genome Biology and Evolution Volume: - Issue: 4 Pages: 1267-1278
  • DOI: 10.1093/gbe/evw063
  • Peer Reviewed / Open Access
• [Presentation] RAD-seqによるシロウオ太平洋型と日本海型の交雑集団の遺伝解析 (2017)
  • Author(s): 平瀬祥太朗，小北智之，永野 惇，菊池 潔
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都港区）
  • Year and Date: 2017-03-26
• [Presentation] 新たなゲノム構成を有する交雑集団の形成：アコハゼのRAD-seq解析 (2016)
  • Author(s): 平瀬祥太朗，手塚あゆみ，永野惇，岩崎渉
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学大岡山キャンパス（東京都目黒区）
  • Year and Date: 2016-08-25
• [Presentation] RAD-Seq analysis of an intertidal goby Chaenogobius annularis uncovered stable hybrid population with novel genomic composition (2016)
  • Author(s): Hirase S, Tezuka A, Nagano A, Iwasaki W.
  • Organizer: The 2016 annual meeting of the Society for the Study of Evolution, the Society of Systematic Biologists, and the American Society of Naturalists
  • Place of Presentation: Austin（United States of America）
  • Year and Date: 2016-06-17
  • Int'l Joint Research
• [Presentation] マハゼの在来地域および侵入地域の分子系統地理 (2016)
  • Author(s): 平瀬祥太朗・Sherrie Chambers・Kathryn Hassell・Melissa Carew・Vincent Pettigrove・征矢野 清・長江真樹・岩崎 渉
  • Organizer: 平成28年度 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学 品川キャンパス
  • Year and Date: 2016-03-26
• [Presentation] How is a novel genetic group generated through hybridization? A RAD-seq analysis of an intertidal goby Chaenogobius annularis (Agohaze) (2016)
  • Author(s): 平瀬祥太朗，手塚あゆみ，永野惇，岩崎渉
  • Organizer: 第16回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学駒場キャンパス（東京都目黒区）
• [Presentation] 日本列島におけるマハゼの分子系統地理および侵入集団の起源の検討 (2015)
  • Author(s): 平瀬祥太朗・岩崎 渉
  • Organizer: 2015年度 日本魚類学会年会
  • Place of Presentation: 近畿大学 奈良キャンパス
  • Year and Date: 2015-09-04