Transcriptome analysis of Japanese eel during metamorphosis induced by starvation
| Project/Area Number |
26850133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Fisheries Research and Education Agency (2015-2016)
Tokyo University of Marine Science and Technology (2014) |
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Principal Investigator |
Sudo Ryusuke 国立研究開発法人水産研究・教育機構, 増養殖研究所, 研究員 (60722676)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | ニホンウナギ / 変態 / トランスクリプトーム解析 / レプトセファルス / 甲状腺ホルモン / 絶食 / RNA-seq / シラスウナギ |
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| Outline of Final Research Achievements |
For understanding of metamorphosis of eels, RNA-seq of head and whole body of eel larvae during metamorphosis was performed. Approximately 373 million reads were generated and assembled 320,321 transcripts. The results of comprehensive analysis of RNA-seq data showed that many genes were drastically changed during the early to late metamorphosis stages. From gene ontology analysis, it is revealed that the expression of genes for transcriptional elongation, regulation of myofibril size and glycolytic process was greatly changed. During metamorphosis, expression of thyroid hormone receptors increased, and thus eel larvae were exposed to thyroxine. All larvae exposed to thyroxine were induced metamorphosis. Therefore, thyroid hormone may also play important role for metamorphosis of eels.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Mapping of quantitative trait loci (QTL) associated with timing of metamorphosis from leptocephali to glass eels in Japanese eel (Anguilla japonica).2016
Author(s)
Nomura K, Fujiwara A, Kai W, Ozaki A, Iwasaki Y, Nakamura Y, Nishiki I, Ojima N, Yasuike M, Sudo R, Tanaka H
Organizer
Plant & Animal Genome Conference XXIV
Place of Presentation
San Diego, CA
Year and Date
2016-01-08
Related Report
Int'l Joint Research
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