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Transcriptome analysis of Japanese eel during metamorphosis induced by starvation

Research Project

Project/Area Number 26850133
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionFisheries Research and Education Agency (2015-2016)
Tokyo University of Marine Science and Technology (2014)

Principal Investigator

Sudo Ryusuke  国立研究開発法人水産研究・教育機構, 増養殖研究所, 研究員 (60722676)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsニホンウナギ / 変態 / トランスクリプトーム解析 / レプトセファルス / 甲状腺ホルモン / 絶食 / RNA-seq / シラスウナギ
Outline of Final Research Achievements

For understanding of metamorphosis of eels, RNA-seq of head and whole body of eel larvae during metamorphosis was performed. Approximately 373 million reads were generated and assembled 320,321 transcripts. The results of comprehensive analysis of RNA-seq data showed that many genes were drastically changed during the early to late metamorphosis stages. From gene ontology analysis, it is revealed that the expression of genes for transcriptional elongation, regulation of myofibril size and glycolytic process was greatly changed. During metamorphosis, expression of thyroid hormone receptors increased, and thus eel larvae were exposed to thyroxine. All larvae exposed to thyroxine were induced metamorphosis. Therefore, thyroid hormone may also play important role for metamorphosis of eels.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Environmental factors affecting the onset of spawning migrations of Japanese eels (Anguilla japonica) in Mikawa Bay Japan2017

    • Author(s)
      Sudo Ryusuke, Akihiro Okamura, Nobuto Fukuda, Michael J. Miller and Katsumi Tsukamoto
    • Journal Title

      Environmental Biology Fishes

      Volume: 100 Issue: 3 Pages: 237-249

    • DOI

      10.1007/s10641-017-0575-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiles of thyroid hormones and mRNA expression for thyroid stimulating hormone in Japanese eel during downstream migration2015

    • Author(s)
      Sudo R, Okamura A, Tsukamoto K
    • Journal Title

      Coastal Marine Science

      Volume: 38 Pages: 1-7

    • NAID

      120005627712

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Migratory restlessness an the role of androgen for increasing behavioral drive in the spawning migration of the Japanese eel2015

    • Author(s)
      Sudo R, Tsukamoto K
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 17430-17430

    • DOI

      10.1038/srep17430

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in the role of the thyroid axis during the metamorphosis of the Japanese eel, Anguilla japonica.2014

    • Author(s)
      Sudo R, Okamura A, Kuroki M, Tsukamoto K.
    • Journal Title

      Journal of Experimental Zoology

      Volume: (掲載受理済み) Issue: 5 Pages: 357-364

    • DOI

      10.1002/jez.1862

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Artificial seedlings production of the Japanese eel2016

    • Author(s)
      Ryusuke Sudo, Kazuharu Nomura, Hideki Tanaka
    • Organizer
      EELRIC
    • Place of Presentation
      Wageningen, the Netherlands.
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 組換えウナギ成長ホルモンの経口投与がニホンウナギ仔魚の成長に及ぼす影響2016

    • Author(s)
      須藤竜介、風藤行紀、野村和晴、田中秀樹
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Mapping of quantitative trait loci (QTL) associated with timing of metamorphosis from leptocephali to glass eels in Japanese eel (Anguilla japonica).2016

    • Author(s)
      Nomura K, Fujiwara A, Kai W, Ozaki A, Iwasaki Y, Nakamura Y, Nishiki I, Ojima N, Yasuike M, Sudo R, Tanaka H
    • Organizer
      Plant & Animal Genome Conference XXIV
    • Place of Presentation
      San Diego, CA
    • Year and Date
      2016-01-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ニホンウナギとAnguilla mossambicaの雑種は仔魚期間が短い2015

    • Author(s)
      須藤竜介、山田祥朗、黒木真理、塚本勝巳、坂本崇.
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-03-27 – 2015-03-31
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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