| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
Intracytoplasmic sperm injection (ICSI) is a useful tool for producing live offspring from immotile sperm. However, the efficiency of fertilization after ICSI is low. It is hypothesized that a failure in oocyte activation causes it in this study. Therefore, this study tried to develop new method for induction of physiological oocyte activation using PLCzeta, which is thought to be the oocyte-activating factor in mammalian sperm. As a result, it was clearly that a lack of PLCzeta in sperm led to a failure in an induction of oocyte activation after ICSI. Furthermore, the injection of PLCzeta mRNA into the pig oocyte injected with sperm induced oocyte activation efficiently and promoted dramatically fertilization. In addition, the induction of oocyte activation using PLCzeta protein will be continued to investigate.
|
