Analysis of carotenoid biosynthesis regulatory mechanisms in lactic acid bacteria
| Project/Area Number |
26850176
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Hagi Tatsuro 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門 畜産物研究領域, 主任研究員 (00510257)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 乳酸菌 / カロテノイド / トランスクリプトーム / メタボローム / 酸化ストレス / 微生物 / 発現制御 / ストレス |
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| Outline of Final Research Achievements |
To clarify the carotenoid-centered oxidative stress response mechanism in lactic acid bacteria (LAB), effects of aerobic culture condition on gene expressions and metabolites in carotenoid-producing Enterococcus gilvus was investigated using whole-transcriptome and metabolome analysis. It turned out that aerobic culture condition increased the level of gene expression of isoprenoid biosynthesis pathway, converting Acetyl-CoA to carotenoid via mevalonate, and metabolites related to Acetyl-CoA biosynthesis. These results indicate that isoprenoid biosynthesis pathway, which could be induced by aerobic condition, plays an important role in oxidative stress response mechanisms in LAB.
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Report
(3 results)
Research Products
(3 results)
