| Project/Area Number |
26850179
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Veterinary medical science
|
|---|
| Research Institution | Hokkaido University (2016)
Oita University (2015)
Obihiro University of Agriculture and Veterinary Medicine (2014) |
|---|
Principal Investigator |
Hayashida Kyoko 北海道大学, 人獣共通感染症リサーチセンター, 博士研究員 (40615514)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | タイレリア / 小型ピロプラズマ / マダニ / 発現解析 / 比較ゲノム / オリエンタリス / 原虫 / スポロゾイト / タイレリアオリエンタリス / ワクチン / SCID-Bo / マダニステージ / Theileria orientalis |
|---|
| Outline of Final Research Achievements |
Inability for in vitro culturing and the lack of animal models are the major obstacles to understand the biology of Theileria orientalis. Especially, research on tick stages of T. orientalis has rarely been undertaken, although such investigations are of paramount importance for the vaccine development targeting sporozoites. In this study, we employed immunodeficient mouse transfused with bovine erythrocyte to infect T. orientalis, and Haemaphysals longicornis larval tick were fed on the infected mice to produce T. orientalis sporozoites in a tick vector. The piroplasm and sporozoites stages were demonstrated in the mouse blood and tick salivary gland, and these data have identified a stage specific expression pattern, which has implication for understanding the biology and breakthrough for selection of vaccine candidate against T. orientalis.
|
