| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The aim of this study was to find a novel mechanism that regulates replication and transcription in rabies virus (RABV) at the RNA level. The RNA motifs conserved among 119 wild-type RABV strains were found at the 5’ end of N CDS and 3’-UTR of M gene. Since the reporter activities using the luciferase mRNAs with the 3’UTR of RABV genes were lower than that of control luciferase mRNA, the 3’-UTR of M and G mRNAs may promote the degradation of M and G mRNAs, respectively. The mini genome reporter assay showed that the synonymous mutations in conserved motif at the 5’ end of N CDS reduced the luciferase activities. In addition, the replication yield of recombinant RABV with synonymous mutations in the conserved motif was lower than that of parental RABV strain. These results suggest that the conserved motif at the 5’ end of N CDS encode nucleotide sequences are required for the effective replication of RABV.
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