Identification of a novel mechanism that regulates replication and transcription at the RNA level in wild-type rabies virus
Project/Area Number |
26850187
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
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Research Institution | Nihon University |
Principal Investigator |
SUZUKI Yuki 日本大学, 生物資源科学部, 助教 (30712492)
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Research Collaborator |
INOUE Satoshi
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 狂犬病ウイルス / 複製・転写制御 / 3' UTR / RNAモチーフ / N遺伝子 / 高度保存領域 / 複製・転写調節 / RNA結合蛋白質 / UTR |
Outline of Final Research Achievements |
The aim of this study was to find a novel mechanism that regulates replication and transcription in rabies virus (RABV) at the RNA level. The RNA motifs conserved among 119 wild-type RABV strains were found at the 5’ end of N CDS and 3’-UTR of M gene. Since the reporter activities using the luciferase mRNAs with the 3’UTR of RABV genes were lower than that of control luciferase mRNA, the 3’-UTR of M and G mRNAs may promote the degradation of M and G mRNAs, respectively. The mini genome reporter assay showed that the synonymous mutations in conserved motif at the 5’ end of N CDS reduced the luciferase activities. In addition, the replication yield of recombinant RABV with synonymous mutations in the conserved motif was lower than that of parental RABV strain. These results suggest that the conserved motif at the 5’ end of N CDS encode nucleotide sequences are required for the effective replication of RABV.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.2016
Author(s)
Horie M, Kobayashi Y, Honda T, Fujino K, Akasaka T, Kohl C, Wibbelt G, Muhldorfer K, Kurth A, Muller MA, Corman VM, Gillich N, Suzuki Y, Schwemmle M, Tomonaga K.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 25873-25873
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around Moukalaba-Doudou National Park (Gabon).2016
Author(s)
Nze-Nkogue C, Horie M, Fujita S, Ogino M, Kobayashi Y, Mizukami K, Masatani T, Ezzikouri S, Matsuu A, Mizutani T, Ozawa M, Yamato O, Ngomanda A, Yamagiwa J, Tsukiyama-Kohara K
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Journal Title
Virus Genes
Volume: 52
Issue: 5
Pages: 671-678
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Computational and molecular analysis of conserved influenza A virus RNA secondary structures involved in infectious virion production2016
Author(s)
Kobayashi, Y.(新姓:鈴木), Dadonaite, B., Doremalen, N., Suzuki, Y., Barclay, W., and Pybus, O.G.
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Journal Title
RNA Biology
Volume: 13
Issue: 9
Pages: 883-894
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Molecular analysis of conserved RNA secondary structures predicted in the packaging signals of influenza A virus M segment2016
Author(s)
Kobayashi, Y.(新姓:鈴木), Dadonaite, B., Dorenalan, N., Suzuki, Y., Barclay, W., and Pybus O.G.
Organizer
第64回日本ウイルス学会
Place of Presentation
札幌コンベンションセンター(北海道・札幌市)
Year and Date
2016-10-23
Related Report
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[Presentation] ハンドウイルカにおけるMRPの発現解析2016
Author(s)
新田野乃, 小林由紀(新姓:鈴木), 小林淳也, 瀬川太雄, 猪島康雄, 前田健, 鈴木美和, 鯉江洋, 伊藤琢也
Organizer
第159回日本獣医学会学術会
Place of Presentation
日本大学生物資源科学部(神奈川県・藤沢市)
Year and Date
2016-09-06
Related Report
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