Single nucleotide polymorphism analysis of cryptorchidism-related genes and establishment of gene diagnosis method for cryptorchidism in dogs

Research Project

Project/Area Number	26850202
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Veterinary medical science
Research Institution	Nippon Veterinary and Life Science University
Principal Investigator	Kobayashi Masanori 日本獣医生命科学大学, 獣医学部, 講師 (80600428)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	一塩基多型 / 犬 / 潜在精巣 / RXFP2
Outline of Final Research Achievements	Cryptorchidism is a disorder in which unilateral or bilateral testes fail to descend into the scrotum. The inheritance of canine cryptorchidism involves genetic (recessive or multifactorial inheritance), epigenetic, and environmental factors. In this study, we examined the relationship between single nucleotide polymorphisms (SNPs) of relaxin/insulin-like family peptide receptor 2 (RXFP2), which plays an important role in testicular descent, and the inheritance of cryptorchidism. As a result, it was suggested that SNPs related to the inheritance of cryptorchidism were not present from 5’-end to intron (int) 1, from exon (ex) 3 to int 4, int 6, from int 7 to int 9, from 11 to int 18.