| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Using a mouse dextran sodium sulfate (DSS)-induced colitis model, we found that commensal Gram-positive bacteria trigger the mobilization of inflammatory monocyte and macrophage. TNF is a representative inflammatory cytokine that aggravates colitis and a target for therapy, predominantly produced by monocyte and macrophage. Interestingly, pretreating mice with vancomycin, which eliminated Gram-positive bacteria significantly reduced the severity of colitis, evaluated by the colon length, cytokine level, histological analysis etc.
In addition, 16S rRNA analysis showed that vancomycin treatment dramatically reduced Lachnospiraceae, the most abundant order of Clostridiales in untreated control mice. We also suggested that this strain stimulated the local production of chemokine by colonic epithelial cells, which induce the mobilization of monocyte/macrophage into the inflamed colon.
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