| Project/Area Number |
26850208
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
Horie Masayuki 鹿児島大学, 共同獣医学部, 特任助教 (20725981)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Yuki 日本大学, 生物資源科学部, 助教 (30712492)
AKASAKA Takumi 帯広畜産大学, 畜産学部, 助教 (40748357)
OGAWA Haruko 帯広畜産大学, 動物・食品衛生研究センター, 教授 (10400079)
IMAI Kunitoshi 帯広畜産大学, 動物・食品衛生研究センター, 教授 (70374767)
TOMONAGA Keizo 京都大学, ウイルス研究所, 教授 (10301920)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 内在性ウイルス様エレメント / 共進化 / RNA依存性RNAポリメラーゼ / ボルナウイルス / 内在化 |
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| Outline of Final Research Achievements |
Endogenous bornavirus-like L (EBLL) elements are heritable sequences derived from bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in eukaryotic genomes. Here, we showed that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of more than 5,000 nucleotides. Sequence analysis revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analyses showed that natural selection operated on eEBLL-1 during the evolution of Eptesicus. We detected efficient transcription of eEBLL-1 in tissues from Eptesicus bats and in cell culture from E. nilssonii that we established. To our knowledge, this study is the first report showing that the eukaryotic genome has gained a riboviral polymerase gene from an ancient virus that has the potential to encode a functional RdRp.
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