An RNA-dependent RNA polymerase gene in the genomes of bats
| Project/Area Number |
26850208
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
Horie Masayuki 鹿児島大学, 共同獣医学部, 特任助教 (20725981)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Yuki 日本大学, 生物資源科学部, 助教 (30712492)
AKASAKA Takumi 帯広畜産大学, 畜産学部, 助教 (40748357)
OGAWA Haruko 帯広畜産大学, 動物・食品衛生研究センター, 教授 (10400079)
IMAI Kunitoshi 帯広畜産大学, 動物・食品衛生研究センター, 教授 (70374767)
TOMONAGA Keizo 京都大学, ウイルス研究所, 教授 (10301920)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 内在性ウイルス様エレメント / 共進化 / RNA依存性RNAポリメラーゼ / ボルナウイルス / 内在化 |
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| Outline of Final Research Achievements |
Endogenous bornavirus-like L (EBLL) elements are heritable sequences derived from bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in eukaryotic genomes. Here, we showed that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of more than 5,000 nucleotides. Sequence analysis revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analyses showed that natural selection operated on eEBLL-1 during the evolution of Eptesicus. We detected efficient transcription of eEBLL-1 in tissues from Eptesicus bats and in cell culture from E. nilssonii that we established. To our knowledge, this study is the first report showing that the eukaryotic genome has gained a riboviral polymerase gene from an ancient virus that has the potential to encode a functional RdRp.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus2016
Author(s)
Masayuki Horie, Yuki Kobayashi, Tomoyuki Honda, Kan Fujino, Takumi Akasaka, Claudia Kohl, Gudrun Wibbelt , Kristin Muehldorfer, Andreas Kurth, Marcel A. Mueller, Victor M. Corman, Nadine Gillich, Yoshiyuki Suzuki, Martin Schwemmle, Keizo Tomonaga
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Journal Title
Scientific Reports
Volume: In press
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] ボルナウイルス感染症2016
Author(s)
堀江真行
Organizer
第89回鹿児島県家畜疾病診断会
Place of Presentation
鹿児島大学（鹿児島県鹿児島市）
Year and Date
2016-02-23
Related Report
Invited
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[Presentation] A putative RNA-dependent RNA polymerase gene derived from an ancient bornavirus in bats2015
Author(s)
Masayuki Horie, Yuki Kobayashi, Tomoyuki Honda, Takumi Akasaka, Nadine Gillich, Marcel A. Mueller, Victor M. Corman, Haruko Ogawa, Kunitoshi Imai, Yoshiyuki Suzuki, Martin Schwemmle, Keizo Tomonaga
Organizer
第63回日本ウイルス学会学術集会
Place of Presentation
福岡国際会議場（福岡県福岡市）
Year and Date
2015-11-22
Related Report
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