Transglutaminases might be involved in lipopolysaccharide-induced glial activation.
| Project/Area Number |
26850209
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Takano Katsura 大阪府立大学, 生命環境科学研究科(系), 准教授 (50453139)
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| Research Collaborator |
KAWABE KENJI 大阪府立大学, 生命環境科学研究科, 博士課程大学院生
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | グリア細胞 / トランスグルタミナーゼ / アストロサイト / ミクログリア / 一酸化窒素 / エンドサイトーシス |
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| Outline of Final Research Achievements |
Activation of glia has been observed in neurodegenerative diseases. Engulfment of neurons by activated microglia may play roles in the pathogenesis of neurodegeneration. Transglutaminase 2 (TG2) is a cross-linking enzyme, which is activated in neurodegenerative diseases. We previously demonstrated in cultured astrocytes that the expression of TG2 was increased by lipopolysaccharide (LPS)-stimulation. LPS-induced NO production was inhibited by cystamine (inhibitor of TG activity); suggesting that TG2 induction in the activated astrocytes may associate with NO production. In the present study, we examined the expression of TG2 in mouse microglial cell line BV-2. We found that LPS increased TG2 expression and TG activity also in BV-2 cells. LPS increased the endocytosis in BV-2 cells, and cystamine significantly suppressed the LPS-induced endocytosis. These results suggest that the increased expression of TG2 in activated microglia might be involved in the mechanism of their endocytosis.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Mechanism for increased hepatic glycerol synthesis in the citrin/mitochondria glycerol-3-phosphate dehydrogenase double-knockout mouse: Urine glycerol and glycerol 3-phosphate as potential diagnostic markers of human citrin deficiency.2015
Author(s)
Moriyama M, Fujimoto Y, Rikimaru S,Ushikai M, Kuroda E, Kawabe K, Takano K,Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Okano Y, Yazaki M, Ikeda SI,Zhang C, Song YZ,Sakamoto O, Kure S, Mitsubuchi H, Endo F, Horiuchi M, Nakamura Y, Yamamura KI, Saheki T.
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Journal Title
Biochim Biophys Acta.
Volume: 印刷中
Issue: 9
Pages: 1787-1795
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] A dibenzoylmethane derivative inhibits lipopolysaccharide-induced NO production.2016
Author(s)
Katsura Takano, Kumiko Sugita, Natsumi Ishida, Mitsuaki Moriyama, Satoshi Hibino, Tominari Choshi, Rika Murakami, Masashi Yamada, Hiroto Suzuki, Osamu Hori, Yoichi Nakamura
Organizer
第89回日本薬理学会年会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2016-03-09
Related Report
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