| Project/Area Number |
26850211
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | University of Toyama |
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Principal Investigator |
MIZUSHIMA Shusei 富山大学, 大学院理工学研究部(工学), 特命助教 (20515382)
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| Co-Investigator(Renkei-kenkyūsha) |
SASANAMI Tomohiro 静岡大学, 大学院農学研究科, 准教授 (80322139)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 体細胞クローン家禽 / 雌性核 / 胚盤葉細胞 / 皮膚細胞 / 筋原細胞 / 近縁種 / 顕微授精法 / 卵子活性化 / 体細胞クローン動物 / ウズラ / 卵割球細胞 / 鳥類卵 / クローン胚 / 胚発生 / 卵賦活化 / フォスフォリパーゼCzeta / アコニット酸ヒドラターゼ / クエン酸合成酵素 |
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| Outline of Final Research Achievements |
Somatic cell-nuclear transfer (SCNT) becomes a powerful tool to make copies of individuals in many mammalian species, but in birds no SCNT has been reported because physiological polyspermic egg-activation leading to meiotic resumption and subsequent embryo development is difficult to mimic in vitro. In the present study, I show (1) sperm extracts contain three factors- phospholipase Czeta (PLCZ), aconitate hydratase (AH) and citrate synthase (CS)- essential to complete activation of quail egg, and (2) the first case of live quail chick production by intracytoplasmic sperm injection using cRNAs mixture of PLCZ, AH and CS. Furthermore, I show the embryo development of quail egg microinjected with a nucleus of chicken blastodermal or skin cells together with cRNAs mixture of PLCZ, AH and CS. These results create new opportunities to assist in the production of genetically modified and cloned birds.
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