| Project/Area Number |
26850213
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 再生医療 / 骨治癒 / 皮膚線維芽細胞 / ウマ |
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| Outline of Final Research Achievements |
In this study, dermal fibroblasts with greater differentiation capacities were successfully isolated by flowcytometry technique and three antibodies. When these selected cells were cultured with osteogenic medium and BMP-containing medium, significantly higher expression of osteogenic genes and greater numbers of mineralized nodules by Von-Kossa staining were demonstrated compared to the unselected cells. These results indicate that rapid bone regeneration can be achieved by selecting skin cells with high differentiation capacities and locally administering these cells with BMP proteins.
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