| Project/Area Number |
26850214
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
YOSHIDA Naoko 関西医科大学, 医学部, 講師 (40392170)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ノックインマウス / 膵内分泌前駆細胞 / 膵臓発生 / 膵臓 / 内分泌前駆細胞 / β細胞 / 細胞系譜追跡実験 / 発生生物学 / 発生・分化 / 細胞・組織 / 再生医学 / 糖尿病 |
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| Outline of Final Research Achievements |
Pancreatic disease causes diabetes and affects more than 7 million people in Japan, but the mechanism of pancreas formation is not well understood. Here, we attempted to create a new genetically modified mouse (Ngn3-Venus-CreERT2 knock-in mouse) to ask how different cells come together and specialize during development to produce key pancreatic structures called islets of Langerhans. Taking advantage of this knock-in mouse, we will use an approach called 'lineage tracing' that introduces different fluorescent colors into precursor cells. The fluorescence will allow us to follow the cell fate and see what they become during pancreas development on a molecular level. Moreover, the present study sought to establish 3D culture system to produce pancreatic structures. We obtained some promising results which would be useful for future drug screening and regenerative medicine.
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