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Understanding of insect hormone traffic using FRET-based biosensor

Research Project

Project/Area Number 26850216
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Kikuta Shingo  東京農工大学, (連合)農学研究科(研究院), 助教 (90718686)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsFRET / 分子イメージング / 遺伝子導入 / バイオセンサー
Outline of Final Research Achievements

The purpose of this study is to introduce a FRET-based juvenile hormone sensor into cells and express it to obtain the fluorescent signals.
1) culture cell level, change in cytoplasmic fluorescence signals caused by the sensor after JH addition. The results indicate that JH was incorporated across transmembtrane unrelated to transporters or mediation by receptors.
2) insect tissue level, introduction of a transient sensor gene to silkworm larvae could be achieved, but it was not possible to introduce it to fat body which was originally planned. It was thought that this was due to the problem of introduction efficiency due to the insulation of the fat body.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] FRET sensor-based quantification of intracellular trehalose in mammalian cells2016

    • Author(s)
      Shingo Kikuta, Bi-Huei Hou, Ryoichi Sato, Wolf B. Frommer, Takahiro Kikawada
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 1 Pages: 162-165

    • DOI

      10.1080/09168451.2015.1069699

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A novel functional glucose transporter in the white shrimp Litopenaeus vannamei -LvGLUT2- is up-regulated during hypoxia in hepatopancreas.2015

    • Author(s)
      José A. Martínez-Quintana, Shingo Kikuta, Monserrath Felix-Portillo, Alma B. Peregrino-Uriarte, Gloria Yepiz-Plascencia
    • Journal Title

      Marine Environmental Research

      Volume: 112 Pages: 61-67

    • DOI

      10.1016/j.marenvres.2015.09.007

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Herbivory-induced glucose transporter gene expression in the brown planthopper, Nilaparvata lugens.2015

    • Author(s)
      *Shingo Kikuta, Yuki Nakamura, Makoto Hattori, Ryoichi Sato, Takahiro Kikawada, *Hiroaki Noda
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 64 Pages: 60-67

    • DOI

      10.1016/j.ibmb.2015.07.015

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Construction of Optimized CRISPR/Cas System to Reveal the Mechanisms of Anhydrobiosis in the Sleeping Chironomid.2015

    • Author(s)
      Jun Okada, Shingo Kikuta, Oleg Gusev, Yoshitaka Suetsugu, Richard Cornette, Tetsushi Sakuma, Takashi Yamamoto, *Takahiro Kikawada
    • Journal Title

      Cryobiology and Cryotechnology

      Volume: 61 Pages: 69-73

    • NAID

      110009959817

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki2015

    • Author(s)
      Rie Hatanaka, Oleg Gusev, Richard Cornette, Sachiko Shimura, Shingo Kikuta, Jun Okada, Takashi Okuda,Takahiro Kikawada
    • Journal Title

      Planta

      Volume: - Issue: 2 Pages: 451-459

    • DOI

      10.1007/s00425-015-2284-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparative genome sequencing reveals genomic signature of desiccation tolerance in the anhydrobiotic midge2014

    • Author(s)
      Gusev O. Okuda T, Kikawada T etal.,
    • Journal Title

      Nat. Commun

      Volume: 5 Issue: 1 Pages: 1-9

    • DOI

      10.1038/ncomms5784

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin-like receptor by directed evolution based on phage display and biopanning selections of domain II loop 2 mutant toxins.2014

    • Author(s)
      79.Haruka Endo, Yuki Kobayashi, Yasushi Hoshino, Shiho Tanaka, Shingo Kikuta,Hiroko Tabunoki & Ryoichi Sato
    • Journal Title

      Microbiology Open

      Volume: 3 Issue: 4 Pages: 568-577

    • DOI

      10.1002/mbo3.188

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ネムリユスリカ由来培養細胞における外来性タンパクの常温乾燥保護能の解析2015

    • Author(s)
      渡辺 俊介, 岡田 淳, 十亀 陽一郎, 佐藤 令一, 黄川田 隆洋, 菊田 真吾
    • Organizer
      第16回極限環境生物学会年会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2015-11-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 昆虫培養細胞による外来性タンパクの常温乾燥保存2015

    • Author(s)
      渡辺 俊介、佐藤 令一、黄川田 隆洋、菊田 真吾
    • Organizer
      日本農芸化学会関東支部2015年度支部大会
    • Place of Presentation
      お茶の水女子大学(東京都文京区)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] ポリエチレンイミンポリマーにより付着性を高めた昆虫浮遊性細胞の動態観察2015

    • Author(s)
      菊田 真吾・渡辺 俊介・佐藤 令一・黄川田 隆洋
    • Organizer
      第59回日本応用動物昆虫学会大会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ネムリユスリカ由来培養細胞の乾燥耐性と細胞周期の停止2015

    • Author(s)
      渡辺 俊介・菊田 真吾・佐藤 令一・畑中 理恵・グセフ オレグ・奥田 隆・黄川田 隆洋
    • Organizer
      第59回日本応用動物昆虫学会大会
    • Place of Presentation
      山形大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Remarks] 個人HPページ

    • URL

      http://web.tuat.ac.jp/~singo_61/SingoKikuta/HOME.html

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 幼若ホルモンセンサー2015

    • Inventor(s)
      山崎俊正 塩月孝博 黄川田隆洋 土屋渉 菊田真吾
    • Industrial Property Rights Holder
      山崎俊正 塩月孝博 黄川田隆洋 土屋渉 菊田真吾
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-03-02
    • Acquisition Date
      2016-09-05
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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