Understanding of insect hormone traffic using FRET-based biosensor
| Project/Area Number |
26850216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Kikuta Shingo 東京農工大学, (連合)農学研究科(研究院), 助教 (90718686)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | FRET / 分子イメージング / 遺伝子導入 / バイオセンサー |
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| Outline of Final Research Achievements |
The purpose of this study is to introduce a FRET-based juvenile hormone sensor into cells and express it to obtain the fluorescent signals.
1) culture cell level, change in cytoplasmic fluorescence signals caused by the sensor after JH addition. The results indicate that JH was incorporated across transmembtrane unrelated to transporters or mediation by receptors.
2) insect tissue level, introduction of a transient sensor gene to silkworm larvae could be achieved, but it was not possible to introduce it to fat body which was originally planned. It was thought that this was due to the problem of introduction efficiency due to the insulation of the fat body.
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Report
(4 results)
Research Products
(13 results)
