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Visualization and functional analysis of sex pheromone neural circuit of insects using an activity-dependent gene expression

Research Project

Project/Area Number 26850218
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionKanazawa University

Principal Investigator

Taketoshi Kiya  金沢大学, 自然システム学系, 准教授 (90532309)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords初期応答遺伝子 / 神経活動 / フェロモン / 昆虫 / カイコ / ショウジョウバエ / 脳 / Hr38 / 性行動 / fruitless
Outline of Final Research Achievements

How do animals select their behavior in response to certain sensory inputs? Appropriate response to sensory information is critical for animal survival. In the present study, I focused on the brain of insects which has low complexity, but has similar circuit property and molecular basis as the brain of other animals including vertebrates. In many insects, sex pheromone induces stereotypic courtship behavior with clear relationship between receptor and behavior. Here, I established a novel neural circuit tracing method utilizing an activity-dependent gene expression. Recently, I identified a novel immediate early gene Hr38, whose expression increases in response to neural activity. In the present study, I used the promoter activity of Hr38 to express GFP in order to visualize neurons activated by sex pheromone or courtship behavior. In addition to previously known neural circuits, I identified novel circuits involved in courtship behavior.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Invited: 4 results) Book (2 results)

  • [Journal Article] Visualization of neural activity related to instinct behavior in insect brains using a conserved immediate early gene, Hr382015

    • Author(s)
      木矢剛智
    • Journal Title

      生化学

      Volume: 87 Issue: 2 Pages: 221-224

    • DOI

      10.14952/SEIKAGAKU.2015.870221

    • NAID

      40020461590

    • ISSN
      0037-1017
    • Year and Date
      2015-04-25
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori.2014

    • Author(s)
      Taketoshi Kiya, Koudai Morishita, Keiro Uchino, Masafumi Iwami, and Hideki Sezutsu
    • Journal Title

      PLoS One

      Volume: 9 (11) Issue: 11 Pages: e113156-e113156

    • DOI

      10.1371/journal.pone.0113156

    • NAID

      120005537889

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Splicing variants of NOL4 differentially regulate the transcription activity of Mlr1 and Mlr2 in cultured cells.2014

    • Author(s)
      Seika Takayanagi-Kiya, Kayo Misawa-Hojo, Takstoshi Kiya, Takekazu Kunieda, Takeo Kubo
    • Journal Title

      Zool. Sci.

      Volume: 31(11) Issue: 11 Pages: 735-740

    • DOI

      10.2108/zs140049

    • NAID

      40020278707

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ショウジョウバエ脳における活動依存的な神経回路可視化法の確立による性フェロモン情報を統合する脳領域の同定と機能解析2015

    • Author(s)
      木矢 剛智、岩見 雅史
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Hr38の神経活動依存的発現を利用した、カイコガの脳において性フェロモンに応答する神経回路の可視化2015

    • Author(s)
      山田 裕果, 原 千穂, 内野 恵郎, 瀬筒 秀樹, 岩見 雅史, 木矢 剛智
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 初期応答遺伝子Hr38はCREBの下流遺伝子として発現し、キイロショウジョウバエの長期求愛記憶の形成を促進する2015

    • Author(s)
      箕口昌杜、大村知世、長田有加、佐藤翔馬、坂井貴臣、岩見雅史、木矢剛智
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 昆虫の生得的行動の神経基盤:活動依存的な神経回路可視化法の開発によるアプローチ2015

    • Author(s)
      木矢剛智
    • Organizer
      平成 27 年度 蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      札幌 北海道大学農学部
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 昆虫の生得的行動の神経基盤:活動依存的な神経回路可視化法の開発によるアプローチ2015

    • Author(s)
      木矢剛智
    • Organizer
      日本動物学会北海道支部第562回支部講演会
    • Place of Presentation
      札幌 北海道大学理学部
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳において性行動時に活動した神経回路の可視化2015

    • Author(s)
      木矢 剛智、岩見 雅史
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳においてメスに反応する神経細胞の検出2014

    • Author(s)
      木矢 剛智、佐藤 優希、岩見 雅史
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、横浜、神奈川
    • Year and Date
      2014-11-24 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Hr38の神経活動依存的発現を利用した、カイコガの脳において性フェロモンに応答する神経回路の可視化2014

    • Author(s)
      山田 裕果, 原 千穂, 内野 恵郎, 櫻井 健志, 神崎 亮平, 瀬筒 秀樹, 岩見 雅史, 木矢 剛智
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、横浜、神奈川
    • Year and Date
      2014-11-24 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 初期応答遺伝子Hr38はショウジョウバエの長期記憶において重要な役割を果たす2014

    • Author(s)
      箕口昌杜、大村知世、長田有加、佐藤翔馬、坂井貴臣、岩見雅史、木矢剛智
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜、横浜、神奈川
    • Year and Date
      2014-11-24 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 昆虫の生得的行動の神経基盤:活動依存的な神経回路可視化法の開発によるアプローチ2014

    • Author(s)
      木矢 剛智
    • Organizer
      日本動物学会中部支部会
    • Place of Presentation
      金沢大学臨海実験所、能登、石川
    • Year and Date
      2014-11-22 – 2014-11-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Visualization of neural activity of forager honeybee brain by IEGs2014

    • Author(s)
      Taketoshi Kiya
    • Organizer
      International Union for the Study of Social Insects
    • Place of Presentation
      Cairns Convention Centre, Queensland, Australia
    • Year and Date
      2014-07-13 – 2014-07-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Detection of neurons that respond to female in the brain of male Drosophila, using a neural activity marker gene, Hr382014

    • Author(s)
      Taketoshi Kiya, Masafumi Iwami
    • Organizer
      JDRC11
    • Place of Presentation
      金沢歌劇座、金沢、石川
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Book] 研究者が教える動物実験 第2巻:神経・筋 26章・27章2015

    • Author(s)
      木矢剛智
    • Total Pages
      9
    • Publisher
      共立出版
    • Related Report
      2015 Annual Research Report
  • [Book] 生化学誌87巻2号2015

    • Author(s)
      木矢 剛智
    • Total Pages
      4
    • Publisher
      公益社団法人日本生化学会
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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