Construction and analysis of recombinant cyanobacterium expressing a chimeric enzyme complex for ethylene production
| Project/Area Number |
26850224
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental agriculture(including landscape science)
|
|---|
| Research Institution | Meijo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TAMURA Hiroto 名城大学, 農学部, 教授 (90267972)
HOSODA Akifumi 名城大学, 農学部, 准教授 (50434618)
|
|---|
| Research Collaborator |
Bayer Edward A.
Raphael Lamed
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | エチレン / シアノバクテリア / 酵素複合体 / Synechococcus / コヘシン / ドックリン / ACC合成酵素 / ACC酸化酵素 / セルロソーム / コヘシン-ドックリン |
|---|
| Outline of Final Research Achievements |
To reduce the rate of global warming, we have developed a novel bio-ethylene synthetic process using the both cellulosomal system and cyanobacteria. In vivo synergistic effect of chimeric enzyme complex on ethylene production was demonstrated. In order to produce more bio-ethylene from cyanobacteria, the investigation was performed as follows. First, to produce ethylene from SAM via ACC in a concerted fashion with less inhibition of ACS, we have constructed two chimeric enzyme complexes, SOC3D1 and SOC3D2, based on cellulosomal system. Second, two chimeric enzyme complexes were introduced into Synechococcus elongatus PCC7942. Then, we have examined the in vivo ethylene accumulation of engineered S. elongatus PCC7942 cells to determine whether the expressed chimeric enzymes produce more ethylene.
|
Report
(4 results)
Research Products
(12 results)
-
[Journal Article] Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions2017
Author(s)
Vered Israeli-Ruimy, Pedro Bule, Sadanari Jindou, Bareket Dassa, Sarah Moras, Ilya Borovok, Yoav Barak, Michal Slutzki, Yuval Hamberg, Vânia Cardoso, Victor D. Alves, Shabir Najmudin, Bryan A. White, Harry J. Flint, Harry J. Gilbert, Raphael Lamed, Carlos M. G. A. Fontes & Edward A. Bayer
-
Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42355-42355
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Standalone cohesin as a molecular shuttle in cellulosome assembly.2015
Author(s)
Voronov-Goldman M, Yaniv O, Gul O, Yoffe H, Salama-Alber O, Slutzki M, Levy-Assaraf M, Jindou S, Shimon LJ, Borovok I, Bayer EA, Lamed R, Frolow F.
-
Journal Title
FEBS Letters
Volume: 589
Issue: 14
Pages: 1569-1579
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
