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Genetic and biochemical characterization of cellulases from nobel deep-sea cellulolytic bacteria

Research Project

Project/Area Number 26850226
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental agriculture(including landscape science)
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Uchimura Kohsuke  国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 技術副主事 (70416006)

Research Collaborator Deguchi Shigeru  
Kobayashi Tohru  
Takaki Yoshihiro  
Tsudome Mikiko  
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsセルラーゼ / 深海
Outline of Final Research Achievements

We have investigated cellulases from deep-sea cellulolytic bacteria. Cellulases used were purified from a culture broth of a deep-sea bacterium or produced by recombinant Escherichia coli harboring cellulase gene(s) derived from genome analysis. Each cellulase degraded cellulose or acid swollen cellulose well under low temperature, weak alkaline region, and the presence of NaCl, nearly like a deep-sea environment. One of the distinguished characters of these cellulases was their higher molecular weights. They were attributed to a glycoprotein or enzyme possessing several unique cellulose-binding domain. Thus, it revealed that the novel cellulolytic bacteria dwelling in deep-sea produce clearly different cellulaeses from those of terrestrial microorganisms.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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