Analysis of plant RNA virus-mediated inhibition of programmed cell death

• Project/Area Number: 26850231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Komatsu Ken 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (60451837)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物ウイルス / 全身壊死

Outline of Final Research Achievements

Plantago asiatica mosaic virus (PlAMV) is a plant RNA virus and induces systemic necrosis in its infected Nicotiana benthamiana plants, which is associated with programmed cell death (PCD). In this project, we constructed PlAMV mutants and analyzed its necrosis-inducing ability, and found that PlAMV with mutation in its coat protein (CP) gene induced more severe necrosis in plants. This enhancement of necrosis-inducing ability is also observed in a PlAMV mutant that possesses leucine (residue in the 3rd position of CP) to alanine (L3A) mutation, which is important for its cell-to-cell movement function. Moreover, although yeast two-hybrid analysis revealed interaction between CP and helicase domain (HEL) of a replicase, which is an elicitor of necrosis, L3A mutation of CP did not abolish this interaction. This implied that CP and HEL interaction is not responsible for the PCD (necrosis) inhibition by CP.

Research Products

• [Journal Article] Detection of Magnaporthe oryzae chrysovirus 1 in Japan and establishment of a rapid, sensitive and direct diagnostic method based on reverse transcription loop-mediated isothermal amplification. (2016)
  • Author(s): Komatsu K, Urayama S, Katoh Y, Fuji S, Hase S, Fukuhara T, Arie T, Teraoka T, Moriyama H(責任著者).
  • Journal Title: Archives of Virology Volume: 161 Issue: 2 Pages: 317-326
  • DOI: 10.1007/s00705-015-2666-x
  • Peer Reviewed / Open Access
• [Journal Article] Genome sequence of a novel victorivirus identified in the phytopathogenic fungus Alternaria arborescens. (2016)
  • Author(s): Komatsu K., Katayama Y., Omatsu T., Mizutani T., Fukuhara T., Kodama M., Arie T., Teraoka T., Moriyama H.
  • Journal Title: Archives of Virology Volume: 162 Issue: 6 Pages: 1-4
  • DOI: 10.1007/s00705-016-2796-9
  • Peer Reviewed
• [Journal Article] Functional characterization of the principal sigma factor RpoD of phytoplasmas via an in vitro transcription assay. (2015)
  • Author(s): Miura C., Komatsu K., Maejima K., Nijo T., Kitazawa Y., Tomomitsu T., Yusa A., Himeno M., Oshima K., Namba S.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11893-11893
  • DOI: 10.1038/srep11893
  • Peer Reviewed / Open Access
• [Journal Article] A detection method based on reverse transcription loop-mediated isothermal amplification for a genetically heterogeneous plantago asiatica mosaic virus. (2015)
  • Author(s): Komatsu K., Maejima K., Fujita N., Netsu O., Tomomitsu T., Arie T., Teraoka T., Namba S.
  • Journal Title: Journal of General Plant Pathology Volume: 81 Issue: 4 Pages: 297-303
  • DOI: 10.1007/s10327-015-0599-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequence of a Japanese Isolate of <i>Daphne virus S</i> (2015)
  • Author(s): Fujita N, Komatsu K., Neriya Y., Kagiwada S., Hara S., Miyazaki A., Netsu O., Hashimoto M., Yamaji Y., Namba S.
  • Journal Title: CYTOLOGIA Volume: 80 Issue: 3 Pages: 327-330
  • DOI: 10.1508/cytologia.80.327
  • NAID: 130005100888
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed
• [Journal Article] Cell death triggered by a putative amphipathic helix of radish mosaic virus helicase protein is tightly correlated with host membrane modification. (2015)
  • Author(s): Hashimoto M., Komatsu K., Iwai R., Keima T., Maejima K., Shiraishi T., Ishikawa K., Yoshida T., Kitazawa Y., Okano Y., Yamaji Y., Namba S.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 28 Issue: 6 Pages: 675-88
  • DOI: 10.1094/mpmi-01-15-0004-r
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nucleocapsid protein from fig mosaic virus forms cytoplasmic agglomerates that are hauled by endoplasmic reticulum streaming. (2015)
  • Author(s): Ishikawa K., Miura C., Maejima K., Komatsu K., Hashimoto M., Tomomitsu T., Fukuoka M., Yusa A., Yamaji Y., Namba S.
  • Journal Title: Journal of Virology Volume: 89 Issue: 1 Pages: 480-491
  • DOI: 10.1128/jvi.02527-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional comparison of RNA silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus. (2015)
  • Author(s): Netsu O., Hiraguri A., Uehara-Ichiki T., Komatsu K., Sasaya T.
  • Journal Title: Virus Research Volume: 203 Pages: 10-19
  • DOI: 10.1016/j.virusres.2015.03.010
• [Journal Article] Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3' end (2014)
  • Author(s): Yoshida T., Kitazawa Y., Komatsu K., Neriya Y., Ishikawa K., Fujita N., Hashimoto M., Maejima K., Yamaji Y., Namba S
  • Journal Title: Archives of Virology Volume: 159 Issue: 11 Pages: 3161-3165
  • DOI: 10.1007/s00705-014-2175-3
  • Peer Reviewed
• [Journal Article] The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways. (2014)
  • Author(s): Minato, N., Himeno, M., Hoshi, A., Maejima, K., Komatsu, K., Takebayashi, Y., Kasahara, H., Yusa, A., Yamaji, Y., Oshima, K., Kamiya, Y. & Namba, S.
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep07399
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Nicotiana benthamianaのプロトプラストを用いたオオバコモザイクウイルスの複製量の解析 (2016)
  • Author(s): 鈴木健央、有江力、寺岡徹、小松健
  • Organizer: 平成28年度日本植物病理学会本大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] GFP発現ウイルスベクターを用いたプラントアクチベータ―の植物RNAウイルス感染抑制効果の評価系構築 (2016)
  • Author(s): 松尾優希、鈴木健央、有江力、寺岡徹、小松健
  • Organizer: 平成28年度日本植物病理学会本大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] プラントアクチベーターのウイルス感染抑制効果の検定系の確立 (2016)
  • Author(s): 小松健、松尾優希、鈴木健央、寺岡徹、有江力
  • Organizer: 日本農薬学会第41回大会
  • Place of Presentation: 島根大学松江キャンパス
  • Year and Date: 2016-03-18
• [Presentation] イネいもち病菌マイコウイルスMoCV1のRT-LAMP を用いた検出系の確立 (2015)
  • Author(s): 小松健、藤田尚子、有江力、寺岡徹、森山裕充
  • Organizer: 平成27年度日本植物病理学会本大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-31
• [Presentation] 種内ゲノム変異の大きなポテックスウイルス種の RT-LAMP を用いた検出法の確立 (2014)
  • Author(s): 小松健、前島健作、藤田尚子、友光達哉、松田健太郎、山次康幸、有江力、寺岡徹、難波成任
  • Organizer: 平成26年度日本植物病理学会関東部会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2014-09-11
• [Presentation] オオバコモザイクウイルス（plantago asiatica mosaic virus; PlAMV）を用いた外来遺伝子発現ベクターの構築 (2014)
  • Author(s): 小松健、湊菜未、北沢優悟、岡野夕香里、前島健作、山次康幸、難波成任
  • Organizer: 平成26年度日本植物病理学会本大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-06-04