Analysis of plant RNA virus-mediated inhibition of programmed cell death
| Project/Area Number |
26850231
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Komatsu Ken 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (60451837)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 植物ウイルス / 全身壊死 |
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| Outline of Final Research Achievements |
Plantago asiatica mosaic virus (PlAMV) is a plant RNA virus and induces systemic necrosis in its infected Nicotiana benthamiana plants, which is associated with programmed cell death (PCD). In this project, we constructed PlAMV mutants and analyzed its necrosis-inducing ability, and found that PlAMV with mutation in its coat protein (CP) gene induced more severe necrosis in plants. This enhancement of necrosis-inducing ability is also observed in a PlAMV mutant that possesses leucine (residue in the 3rd position of CP) to alanine (L3A) mutation, which is important for its cell-to-cell movement function. Moreover, although yeast two-hybrid analysis revealed interaction between CP and helicase domain (HEL) of a replicase, which is an elicitor of necrosis, L3A mutation of CP did not abolish this interaction. This implied that CP and HEL interaction is not responsible for the PCD (necrosis) inhibition by CP.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Complete Genome Sequence of a Japanese Isolate of <i>Daphne virus S</i>2015
Author(s)
Fujita N, Komatsu K., Neriya Y., Kagiwada S., Hara S., Miyazaki A., Netsu O., Hashimoto M., Yamaji Y., Namba S.
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Journal Title
CYTOLOGIA
Volume: 80
Issue: 3
Pages: 327-330
DOI
NAID
ISSN
0011-4545, 1348-7019
Related Report
Peer Reviewed
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[Journal Article] Cell death triggered by a putative amphipathic helix of radish mosaic virus helicase protein is tightly correlated with host membrane modification.2015
Author(s)
Hashimoto M., Komatsu K., Iwai R., Keima T., Maejima K., Shiraishi T., Ishikawa K., Yoshida T., Kitazawa Y., Okano Y., Yamaji Y., Namba S.
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Journal Title
Molecular Plant-Microbe Interactions
Volume: 28
Issue: 6
Pages: 675-88
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3' end2014
Author(s)
Yoshida T., Kitazawa Y., Komatsu K., Neriya Y., Ishikawa K., Fujita N., Hashimoto M., Maejima K., Yamaji Y., Namba S
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Journal Title
Archives of Virology
Volume: 159
Issue: 11
Pages: 3161-3165
DOI
Related Report
Peer Reviewed
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[Journal Article] The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.2014
Author(s)
Minato, N., Himeno, M., Hoshi, A., Maejima, K., Komatsu, K., Takebayashi, Y., Kasahara, H., Yusa, A., Yamaji, Y., Oshima, K., Kamiya, Y. & Namba, S.
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Journal Title
Scientific reports
Volume: 4
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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