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Elucidation of plant immunity by membrane rafts

Research Project

Project/Area Number 26850232
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionSaitama University

Principal Investigator

NAGANO Minoru  埼玉大学, 理工学研究科, 助教 (80598251)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords膜ラフト / 植物免疫 / スフィンゴ脂質 / イネ / 耐病性 / 植物 / 免疫 / 自然免疫 / ラフト
Outline of Final Research Achievements

In plasma membrane, membrane rafts, in which lipids and proteins accumulate, exist. In this study, I identified immune proteins that transiently exist in membrane rafts during defense responses in rice and analyzed their function. In NADPH oxidases that produce reactive oxygen species, OsRbohB and OsRbohH move to membrane rafts in response to chitin elicitor, and OsRbohB is essential for the resistance to rice blast fungus. In addition, our proteome analysis identified 63 proteins that transiently exist in membrane rafts after the treatment of chitin. In these proteins, I confirmed that 4 proteins localized in the plasma membrane

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (3 results)

  • [Int'l Joint Research] ボルドー大学(フランス)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC 68032016

    • Author(s)
      Ishikawa Y, Miyagi A, Haishima Y, Ishikawa T, Nagano M, Yamaguchi M, Hihara Y, Kawai-Yamada M
    • Journal Title

      Journal of Plant Physiology

      Volume: 205 Pages: 105-112

    • DOI

      10.1016/j.jplph.2016.09.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Plasma membrane microdomains are essential for Rac1-RbohB/H-mediated immunity in rice2016

    • Author(s)
      Nagan M, Ishikawa T, Fujiwara M, Fukao Y, Kawano Y, Kawai-Yamada M, Shimamoto K
    • Journal Title

      Plant Cell

      Volume: 28 Issue: 8 Pages: 1966-1983

    • DOI

      10.1105/tpc.16.00201

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots2015

    • Author(s)
      Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, Nakazono M
    • Journal Title

      Plant Physiology

      Volume: 169 Issue: 1 Pages: 180-193

    • DOI

      10.1104/pp.15.00106

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice.2015

    • Author(s)
      Liu, L., Park, CH., He, F., Nagano, M., Wang, M., Bellizzi, M., Zeng, X., Liu, W., Ning, Y.,Kawano, Y., and Wang, GL.
    • Journal Title

      PLoS Pathog.

      Volume: 11 Issue: 2 Pages: e1004629-e1004629

    • DOI

      10.1371/journal.ppat.1004629

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015

    • Author(s)
      Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 41 Pages: 28569-78

    • DOI

      10.1074/jbc.m114.603282

    • Description
      (in press)(印刷中)
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes2014

    • Author(s)
      Nagano, M., Ishikawa, T., Ogawa, Y., Iwabuchi, M., Nakasone, A., Shimamoto, K., Uchimiya, H., Kawai-Yamada, M.
    • Journal Title

      Planta

      Volume: 240 Issue: 1 Pages: 77-89

    • DOI

      10.1007/s00425-014-2065-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 細胞膜マイクロドメインはイネ免疫を制御する2016

    • Author(s)
      長野稔、石川寿樹、島本功、川合真紀
    • Organizer
      第29回植物脂質シンポジウム
    • Place of Presentation
      大阪大学豊中キャンパス(大阪府豊中市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞膜マイクロドメイン局在型NADPHオキシダーゼはイネ免疫に関与する2016

    • Author(s)
      長野稔、島本功、川合真紀
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター 東北大学川内北キャンパス(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 2-hydroxy sphingolipids are essential for the formation of plasma membrane microdomains and innate immunity in plants2016

    • Author(s)
      Nagano M., Ukawa T., Ishikawa T., Shimamoto K., Kawai-Yamada M.
    • Organizer
      22nd International Symposium on Plant Lipids
    • Place of Presentation
      Goettingen(ドイツ)
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネの細胞膜マイクロドメインに局在するNADPHオキシダーゼは自然免疫に関与する2016

    • Author(s)
      長野稔、島本功、川合真紀
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手県盛岡市 岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 細胞膜マイクロドメインはNADPHオキシダーゼを介して植物免疫を制御する2015

    • Author(s)
      長野稔、石川寿樹、藤原正幸、島本功、川合真紀
    • Organizer
      BMB2015
    • Place of Presentation
      兵庫県神戸市 神戸ポートランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 2-hydroxylation of sphingolipid fatty acids is essential for the formation of plasma membrane microdomains and innate immunity in plants2015

    • Author(s)
      Minoru Nagano, Toshiki Ishikawa, Tomomi Ukawa, Masayuki Fujiwara, Ko Shimamoto, Maki Kawai-Yamada
    • Organizer
      26th International Conference on Arabidopsis Research
    • Place of Presentation
      フランス
    • Year and Date
      2015-07-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] イネ免疫機構における細胞膜マイクロドメイン移行タンパク質の解析2015

    • Author(s)
      長野稔、藤原正幸、深尾陽一朗、島本功、川合真紀
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都・世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Function of plasmamembrane microdomain in plant oxidative stress2015

    • Author(s)
      Maki Kawai-Yamada, Minoru Nagano, Toshiki Ishikawa
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都・世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Sphingolipids are essential for rice innate immunity through the formation of plasma membrane microdomains2015

    • Author(s)
      Minoru Nagano, Toshiki Ishikawa, Masayuki Fujiwara, Yoichiro Fukao, Yoji Kawano, Maki Kawai-Yamada and Ko Shimamoto
    • Organizer
      Gordon Research Conferences, Plant Lipids: Structure, Metabolism & Function
    • Place of Presentation
      Galveston
    • Year and Date
      2015-02-01 – 2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] スフィンゴ脂質が形成する細胞膜マイクロドメインはイネ免疫機構を制御する2014

    • Author(s)
      長野稔、石川寿樹、藤原正幸、深尾陽一朗、河野洋治、川合真紀、島本功
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学(神奈川県・川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Sphingolipid-derived plasma membrane microdomains regulate innate immunity in rice2014

    • Author(s)
      Minoru Nagano, Toshiki Ishikawa, Masayuki Fujiwara, Yoichiro Fukao, Yoji Kawano, Maki Kawai-Yamada and Ko Shimamoto
    • Organizer
      Plant Biology 2014
    • Place of Presentation
      Portland
    • Year and Date
      2014-07-12 – 2014-07-16
    • Related Report
      2014 Research-status Report
  • [Remarks] 埼玉大学理工学研究科・環境制御システムコース 遺伝子環境工学研究室

    • URL

      http://park.saitama-u.ac.jp/~geneenvtech/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 遺伝子環境工学研究室

    • URL

      http://park.saitama-u.ac.jp/~geneenvtech/index.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 戦略的研究部門グリーン・環境領域

    • URL

      http://gr-en.saitama-u.ac.jp/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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