Elucidation of plant immunity by membrane rafts

• Project/Area Number: 26850232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Saitama University
• Principal Investigator: NAGANO Minoru 埼玉大学, 理工学研究科, 助教 (80598251)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膜ラフト / 植物免疫 / スフィンゴ脂質 / イネ / 耐病性 / 植物 / 免疫 / 自然免疫 / ラフト

Outline of Final Research Achievements

In plasma membrane, membrane rafts, in which lipids and proteins accumulate, exist. In this study, I identified immune proteins that transiently exist in membrane rafts during defense responses in rice and analyzed their function.　In NADPH oxidases that produce reactive oxygen species, OsRbohB and OsRbohH move to membrane rafts in response to chitin elicitor, and OsRbohB is essential for the resistance to rice blast fungus. In addition, our proteome analysis identified 63 proteins that transiently exist in membrane rafts after the treatment of chitin. In these proteins, I confirmed that 4 proteins localized in the plasma membrane

Research Products

• [Int'l Joint Research] ボルドー大学(フランス)
• [Journal Article] Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC6893 (2016)
  • Author(s): Ishikawa Y, Miyagi A, Haishima Y, Ishikawa T, Nagano M, Yamaguchi M, Hihara Y, Kawai-Yamada M
  • Journal Title: J. Plant Physiol. Volume: 205 Pages: 105-112
  • DOI: 10.1016/j.jplph.2016.09.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Plasma membrane microdomains are essential for Rac1-RbohB/H-mediated immunity in rice. (2016)
  • Author(s): Nagan M, Ishikawa T, Fujiwara M, Fukao Y, Kawano Y, Kawai-Yamada M, Shimamoto K
  • Journal Title: Plant Cell Volume: 28 Pages: 1966-1983
  • DOI: 10.1105/tpc.16.00201
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ethylene biosynthesis is promoted by very-long-chain fatty acids during lysigenous aerenchyma formation in rice roots. (2015)
  • Author(s): Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, Nakazono M
  • Journal Title: Plant Physiol. Volume: 169 Pages: 180-193
  • DOI: 10.1104/pp.15.00106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice (2015)
  • Author(s): Liu, L., Park, CH., He, F., Nagano, M., Wang, M., Bellizzi, M., Zeng, X., Liu, W., Ning, Y.,Kawano, Y., and Wang, GL.
  • Journal Title: PLoS Pathog Volume: 11
  • DOI: 10.1371/journal.ppat.1004629
  • Peer Reviewed
• [Journal Article] The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase. (2015)
  • Author(s): Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
  • Journal Title: J Biol Chem. Volume: 289 Pages: 28569-28578
  • DOI: 10.1074/jbc.m114.603282
  • Description: (in press)(印刷中)
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes (2014)
  • Author(s): Nagano, M., Ishikawa, T., Ogawa, Y., Iwabuchi, M., Nakasone, A., Shimamoto, K., Uchimiya, H., Kawai-Yamada, M.
  • Journal Title: Planta Volume: 240 Pages: 77-89
  • DOI: 10.1007/s00425-014-2065-7
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 細胞膜マイクロドメインはイネ免疫を制御する (2016)
  • Author(s): 長野稔、石川寿樹、島本功、川合真紀
  • Organizer: 第29回植物脂質シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス（大阪府豊中市）
  • Year and Date: 2016-11-25
• [Presentation] 細胞膜マイクロドメイン局在型NADPHオキシダーゼはイネ免疫に関与する (2016)
  • Author(s): 長野稔、島本功、川合真紀
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] 2-hydroxy sphingolipids are essential for the formation of plasma membrane microdomains and innate immunity in plants (2016)
  • Author(s): Nagano M., Ukawa T., Ishikawa T., Shimamoto K., Kawai-Yamada M.
  • Organizer: 22nd International Symposium on Plant Lipids
  • Place of Presentation: Goettingen（ドイツ）
  • Year and Date: 2016-07-03
  • Int'l Joint Research
• [Presentation] イネの細胞膜マイクロドメインに局在するNADPHオキシダーゼは自然免疫に関与する (2016)
  • Author(s): 長野稔、島本功、川合真紀
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手県盛岡市 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 細胞膜マイクロドメインはNADPHオキシダーゼを介して植物免疫を制御する (2015)
  • Author(s): 長野稔、石川寿樹、藤原正幸、島本功、川合真紀
  • Organizer: BMB2015
  • Place of Presentation: 兵庫県神戸市 神戸ポートランド
  • Year and Date: 2015-12-01
• [Presentation] 2-hydroxylation of sphingolipid fatty acids is essential for the formation of plasma membrane microdomains and innate immunity in plants (2015)
  • Author(s): Minoru Nagano, Toshiki Ishikawa, Tomomi Ukawa, Masayuki Fujiwara, Ko Shimamoto, Maki Kawai-Yamada
  • Organizer: 26th International Conference on Arabidopsis Research
  • Place of Presentation: フランス
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] イネ免疫機構における細胞膜マイクロドメイン移行タンパク質の解析 (2015)
  • Author(s): 長野稔、藤原正幸、深尾陽一朗、島本功、川合真紀
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都・世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Function of plasmamembrane microdomain in plant oxidative stress (2015)
  • Author(s): Maki Kawai-Yamada, Minoru Nagano, Toshiki Ishikawa
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都・世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
  • Invited
• [Presentation] Sphingolipids are essential for rice innate immunity through the formation of plasma membrane microdomains (2015)
  • Author(s): Minoru Nagano, Toshiki Ishikawa, Masayuki Fujiwara, Yoichiro Fukao, Yoji Kawano, Maki Kawai-Yamada and Ko Shimamoto
  • Organizer: Gordon Research Conferences, Plant Lipids: Structure, Metabolism & Function
  • Place of Presentation: Galveston
  • Year and Date: 2015-02-01 – 2015-02-06
• [Presentation] スフィンゴ脂質が形成する細胞膜マイクロドメインはイネ免疫機構を制御する (2014)
  • Author(s): 長野稔、石川寿樹、藤原正幸、深尾陽一朗、河野洋治、川合真紀、島本功
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学（神奈川県・川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
  • Invited
• [Presentation] Sphingolipid-derived plasma membrane microdomains regulate innate immunity in rice (2014)
  • Author(s): Minoru Nagano, Toshiki Ishikawa, Masayuki Fujiwara, Yoichiro Fukao, Yoji Kawano, Maki Kawai-Yamada and Ko Shimamoto
  • Organizer: Plant Biology 2014
  • Place of Presentation: Portland
  • Year and Date: 2014-07-12 – 2014-07-16
• [Remarks] 埼玉大学理工学研究科・環境制御システムコース 遺伝子環境工学研究室
  • URL: http://park.saitama-u.ac.jp/~geneenvtech/index.html
• [Remarks] 遺伝子環境工学研究室
  • URL: http://park.saitama-u.ac.jp/~geneenvtech/index.html
• [Remarks] 戦略的研究部門グリーン・環境領域
  • URL: http://gr-en.saitama-u.ac.jp/