Functional analysis of calcium channels involved in abscisic acid-induced stomatal closure

• Project/Area Number: 26850233
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Okayama University
• Principal Investigator: Munemasa Shintaro 岡山大学, 環境生命科学研究科, 助教 (20641442)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アブシジン酸 / 気孔 / 孔辺細胞 / カルシウム / イオンチャネル / シグナル伝達 / 環境ストレス応答 / シロイヌナズナ

Outline of Final Research Achievements

Guard cell abscisic acid signaling is responsible for drought-triggered stomatal closure response. It has been shown that cytosolic calcium funcions as a second messenger in guard cell abscisic acid signaling. We tried to charactarize calcium channels and calcium sensor kinases that are involved in guard cell abscisic acid signaling in Arabidopsis.

Research Products

• [Int'l Joint Research] University of California, San Diego(米国)
• [Int'l Joint Research] Heidelberg University(Germany)
• [Int'l Joint Research] University of Carthage(Tunisia)
• [Int'l Joint Research] University of California, San Diego(米国)
• [Journal Article] Involvement of OST1 Protein Kinase and PYR/PYL/RCAR Receptors in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis Guard Cells (2016)
  • Author(s): Ye Yin, Yuji Adachi, Yoshimasa Nakamura, Shintaro Munemasa, Izumi C Mori, Yoshiyuki Murata
  • Journal Title: Plant and Cell Physiology Volume: 57 Issue: 8 Pages: 1779-1790
  • DOI: 10.1093/pcp/pcw102
  • NAID: 120006348278
  • Peer Reviewed
• [Journal Article] Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds (2016)
  • Author(s): Lamia Sakouhi, Sondès Rahoui, Marouane Ben Massoud, Shintaro Munemasa, Ezzeddine EL Ferjani, Yoshiyuki Murata, Abdelilah Chaoui
  • Journal Title: Journal of Plant Growth Regulation Volume: 35 Issue: 4 Pages: 1064-1073
  • DOI: 10.1007/s00344-016-9605-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reactive carbonyl species mediate abscisic acid signaling in guard cells. (2016)
  • Author(s): Islam, Md. M., Ye, W., Matsushima, D., Munemasa, S., Okuma, E., Nakamura, Y., Biswas, Md. S., Mano, J. and Murata, Y.
  • Journal Title: Plant & Cell Physiology Volume: 57 Issue: 12 Pages: 2552-2563
  • DOI: 10.1093/pcp/pcw166
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] L-Met Activates Arabidopsis GLR Ca 2+ Channels Upstream of ROS Production and Regulates Stomatal Movement (2016)
  • Author(s): Dongdong Kong, Heng-Cheng Hu, Eiji Okuma, Yuree Lee, Hui Sun Lee, Shintaro Munemasa, Daeshik Cho, Chuanli Ju, Leah Pedoeim, Barbara Rodriguez, Juan Wang, Wonpil Im, Yoshiyuki Murata, Zhen-Ming Pei, June M Kwak
  • Journal Title: Cell Reports Volume: 17 Issue: 10 Pages: 2553-2561
  • DOI: 10.1016/j.celrep.2016.11.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanisms of abscisic acid-mediated control of stomatal aperture (2015)
  • Author(s): Shintaro Munemasa, Felix Hauser, Jiyoung Park, Rainer Waadt, Benjamin Brandt, Julian I Schroeder
  • Journal Title: Current Opinion in Plant Biology Volume: 28 Pages: 154-162
  • DOI: 10.1016/j.pbi.2015.10.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses (2015)
  • Author(s): Rainer Waadt, Bianca Manalansan, Navin Rauniyar, Shintaro Munemasa, Matthew A Booker, Benjamin Brandt, Christian Waadt, Dmitri A Nusinow, Steve A Kay, Hans-Henning Kunz, Karin Schumacher, Alison DeLong, John R Yates, Julian I Schroeder
  • Journal Title: Plant Physiology Volume: 169 Issue: 1 Pages: 760-779
  • DOI: 10.1104/pp.15.00575
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells (2015)
  • Author(s): Benjamin Brandt, Shintaro Munemasa, Cun Wang, Desiree Nguyen, Taiming Yong, Paul G Yang, Elly Poretsky, Thomas F Belknap, Rainer Waadt, Fernando Aleman, Julian I Schroeder
  • Journal Title: Elife Volume: 4
  • DOI: 10.7554/elife.03599
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Open Stomata 1 kinase is essential for yeast elicitor-induced stomatal closure in Arabidopsis (2015)
  • Author(s): Ye W, Adachi Y, Munemasa S, Nakamura Y, Mori IC, Murata Y
  • Journal Title: Plant Cell Physiol Volume: 印刷中 Issue: 6 Pages: 239-248
  • DOI: 10.1093/pcp/pcv051
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diverse Stomatal Signaling and the Signal Integration Mechanism (2015)
  • Author(s): Yoshiyuki Murata, Izumi C Mori, Shintaro Munemasa
  • Journal Title: Annual review of plant biology Volume: 66 Issue: 1 Pages: 369-392
  • DOI: 10.1146/annurev-arplant-043014-114707
  • Peer Reviewed
• [Journal Article] Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana (2014)
  • Author(s): Islam MM, Ye W, Matsushima D, Khokon MA, Munemasa S, Nakamura Y, Murata Y
  • Journal Title: Biosci Biotechnol Biochem Volume: 79 Issue: 1 Pages: 59-62
  • DOI: 10.1080/09168451.2014.951028
  • Peer Reviewed
• [Presentation] グルタチオンによる孔辺細胞アブシシン酸シグナル伝達制御機構の解明 (2017)
  • Author(s): 宗正晋太郎、長橋大樹、横井隆志、中村宜督、村田芳行
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2017-03-17
• [Presentation] ジャスモン酸メチル誘導気孔閉口におけるABA 受容体とOST1 キナーゼの役割 (2017)
  • Author(s): 銀葉、足立優司、中村宜督、宗正晋太郎、森泉、村田芳行
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] パッチクランプ法によるシロイヌナズナ葉緑体膜イオンチャネル活性の直接測定 (2017)
  • Author(s): 宗正晋太郎、中村宜督、村田芳行
  • Organizer: 日本農芸化学会中四国支部第47回講演会（例会）
  • Place of Presentation: 島根大学
  • Year and Date: 2017-01-28
• [Presentation] Salicylic acid signaling in Arabidopsis guard cells (2016)
  • Author(s): Md Yeasin Prodhan, Shintaro Munemasa, Toshiyuki Nakamura, Yoshimasa Nakamura, Yoshiyuki Murata
  • Organizer: 日本農芸化学会2016年度中四国支部大会（第46回講演会）
  • Place of Presentation: 高知大学
  • Year and Date: 2016-09-15
• [Presentation] Guard cell abscisic acid signaling and its interaction with diverse signaling networks (2016)
  • Author(s): Shintaro Munemasa
  • Organizer: International Workshop of Plant Membrane Biology 2016
  • Place of Presentation: Annapolis, MD, USA
  • Year and Date: 2016-06-05
  • Int'l Joint Research / Invited
• [Presentation] Guard cell abscisic acid signaling and its interaction with diverse signaling networks (2016)
  • Author(s): Shintaro Munemasa
  • Organizer: The 17th International Plant Membrane Biology Workshop
  • Place of Presentation: Annapolis, Maryland, USA
  • Year and Date: 2016-06-05
  • Int'l Joint Research / Invited
• [Presentation] シロイヌナズナ孔辺細胞におけるジャスモン酸とエチレンのシグナルクロストーク (2016)
  • Author(s): 宗正 晋太郎, 平尾 友加里, 田浪 かすみ, 中村 宜督, 村田 芳行
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-27
• [Presentation] Ca2+ signaling specificity mechanisms in guard cell ABA signal transduction (2016)
  • Author(s): Shintaro Munemasa, Benjamin Brandt, Yoshiyuki Murata, Julian I Schroeder
  • Organizer: 第57回日本植物生理学会年回シンポジウム「Abscisic acid signaling: Beyond the discovery of PYR/PYL/RCAR」
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
  • Int'l Joint Research / Invited
• [Presentation] 孔辺細胞におけるアブシジン酸シグナル伝達のカルシウム依存性を決定する分子機構の解明 (2016)
  • Author(s): 宗正 晋太郎, Benjamin Brandt, 村田 芳行, Julian I Schroeder
  • Organizer: 日本農芸化学会中四国支部第44回講演会
  • Place of Presentation: 岡山県立大学
  • Year and Date: 2016-01-23
• [Presentation] New Insights into Ca2+-Dependent Abscisic Acid Signalling in Guard Cells (2014)
  • Author(s): Shintaro Munemasa, Benjamin Brandt, Cun Wang, Desiree Nguyen, Julian Schroeder
  • Organizer: PLANT CALCIUM SIGNALING 2014
  • Place of Presentation: Munster, Germany
  • Year and Date: 2014-06-22 – 2014-06-25
  • Invited
• [Book] Regulation of stomatal responses to abiotic and biotic stresses by redox state. Book: Redox State as a Central Regulator of Plant-Cell Stress Responses. Redox State as a Central Regulator of Plant-Cell Stress Responses. Book: Redox State as a Central Regulator of Plant-Cell Stress Responses (2016)
  • Author(s): Yoshiyuki Murata, Shintaro Munemasa, Izumi C. Mori
  • Total Pages: 7
  • Publisher: Springer International Publishing
• [Remarks] Google scholar
  • URL: https://scholar.google.com/citations?user=uBsKlrYAAAAJ&hl=en
• [Remarks] Researchgate
  • URL: https://www.researchgate.net/profile/Shintaro_Munemasa
• [Remarks] Google Scholar Citations
  • URL: http://scholar.google.co.jp/citations?user=uBsKlrYAAAAJ&hl=ja