| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To increase the therapeutic potency of immunostimulatory CpG DNA, we tried to develop polypod-like structured DNA, or polypodna based dendritic nano-sized DDS. We succeeded in constructing dendritic-like DNA (DL-DNA) by connecting polypodnas through their adhesive terminal ends, but not ligating with enzymatic reaction. Furthermore, we found that DL-DNA was efficiently taken up by immune cells, which dramatically increased the cytokine release by CpG DNA. Thus, we have shown that the DL-DNA developed by this approach, can be an useful nano-sized DDS for increasing the potency of nucleic acid drugs.
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