| Project/Area Number |
26860027
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 質量分析 / 重水素標識 / ESI増強誘導体化 / 重水素標識誘導体化 / LC/ESI-MS/MS / 誘導体化 / 高感度化 / 精密定量 / ESI増強原子団 |
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| Outline of Final Research Achievements |
Low assay sensitivity becomes a major problem in the LC/ESI-MS/MS analysis. ESI-enhancing derivatization is useful method for increasing detectability. On the other hands, unavailability of stable isotope-labeled internal standards (ISs) is also a problem. The stable isotope-coded derivatization (ICD) has been recently used as a method to correct the run-to-run ionization differences, including matrix effects, for precise analysis. Based of these backgrounds, we developed novel derivatization reagents to improve these problems simultaneously. We defined these derivatization reagents as ESI-enhancing and deuterium-labeling (EEDL) reagents. We synthesized various type of EEDL reagents.The detectability of EEDL-derivatives were 60-2200-higher than intacts compounds. We also have demonstrated an LC/ESI-MS/MS methods for the quantitative analysis of target metabolites in biological samples. The use of EEDL derivatizations enabled the precise quantification without isotope-coded ISs.
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