Regulation of cell growth and cell viability by polyamines at the level of translation depending on the structural changes of polyamine-RNA complexes
| Project/Area Number |
26860044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Chiba Institute of Science |
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Principal Investigator |
TERUI YUSUKE 千葉科学大学, 薬学部, 准教授 (60433687)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ポリアミン / 細胞増殖 / 細胞生存率 / 翻訳 / 酸化ストレス / CR配列 / 大腸菌 / 動物細胞 / グルタチオン / SoxR / GshA / eEF1A / SD配列 |
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| Outline of Final Research Achievements |
Polyamines exist mostly as polyamine-RNA complexes in cells. It was found that polyamines enhanced the synthesis of specific proteins at the level of translation, which are important for cell growth and cell viability. A group of genes whose expression is enhanced by polyamines at the level of translation be referred to as a “polyamine modulon”.
In this study, we looked for new members of polyamine modulon under oxidative stress conditions in Eschrichia coli. It was found that synthesis of SoxR, EmrR, and GshA proteins was enhanced by polyamines at the level of translation. It was found that synthesis of eEF1A was enhanced by polyamines at the level of translation among various translation factors in mammalian cells. The CR sequence existed in eukaryotic mRNAs, which regulates protein synthetic activity and influences polyamine stimulation of protein synthesis.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Three members of polyamine modulon under oxidative stress conditions: Two transcription factors (SoxR and EmrR) and a glutathione synthase enzyme (GshA)2015
Author(s)
Sakamoto, A., Terui, Y., Yoshida, T., Yamamoto, T., Suzuki, H., Yamamoto, K., Ishihama A., Igarashi, K. and Kashiwagi, K.
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Journal Title
PLoS ONE
Volume: 10
Issue: 4
Pages: e0124883-e0124883
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Role of polyamines under oxidative stress conditions in Escherichia coli.2015
Author(s)
Yoshida T., Sakamoto A., Terui Y., Yamamoto T., Yamamoto K., Ishihama A., Suzuki H., Igarashi K., and Kashiwagi K.
Organizer
Gordon Research Conference on Polyamines
Place of Presentation
Waterville Valley,9 NH, USA
Year and Date
2015-06-14
Related Report
Int'l Joint Research
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[Presentation] ポリアミンによるeIF1A蛋白質合成促進機構2015
Author(s)
坂本明彦, 照井祐介, 吉田健人, 笠原拓馬, 富取秀行, 五十嵐一衛, 柏木敬子
Organizer
日本ポリアミン学会第6回年会
Place of Presentation
東京海洋大学品川キャンパス楽水会館
Year and Date
2015-01-19 – 2015-01-20
Related Report
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[Presentation] 新規アクロレイン毒性軽減細胞の単離と解析2015
Author(s)
植村武史, 中村瑞穂, 坂本明彦, 照井祐介, 富取秀行, 柏木敬子, 五十嵐一衛
Organizer
日本ポリアミン学会第6回年会
Place of Presentation
東京海洋大学品川キャンパス楽水会館
Year and Date
2015-01-19 – 2015-01-20
Related Report
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