Investigatation the roles of GPR4 in myocardial infarction

• Project/Area Number: 26860056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Kyushu University
• Principal Investigator: NAGASAKA AKIOMI 九州大学, 薬学研究科(研究院), 助教 (10723877)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: GPR4 / プロトン感知性受容体 / 心筋梗塞 / ＧＰＲ４

Outline of Final Research Achievements

GPR4 is a receptor which recognizes extracellular protons. Because pH of the heart at the time of myocardial infarction (MI) falls, we produced MI model mice with wild type and GPR4 deficient mice to investigate the roles of GPR4 in MI. We found that GPR4 deficient mice showed a decrease of survival rate and cardiac functions. We also identified GPR4 expressing cells which involved in inflammation after MI.

Research Products

• [Journal Article] Novel functions of GRK6 in macrophages by phosphorylating the non-GPCRs substrates. (2015)
  • Author(s): 1. Akiomi Nagasaka#, Yuki Ohba#, Hitoshi Kurose, & Michio Nakaya*
  • Journal Title: Macrophage Volume: 2
  • DOI: 10.14800/macrophage.1021
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] GRK6 phosphorylates IκBα at Ser32/Ser36 and enhances TNF-α-induced inflammation (2015)
  • Author(s): Yuki Ohba, Michio Nakaya*, Kenji Watari, Akiomi Nagasaka, & Hitoshi Kurose.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 461 Issue: 2 Pages: 307-313
  • DOI: 10.1016/j.bbrc.2015.04.027
  • Peer Reviewed / Open Access
• [Presentation] 心筋梗塞時におけるプロトン感知性受容体Ｔ ｃｅｌｌ ｄｅａｔｈ-ａｓｓｏｃｉａｔｅｄ ｇｅｎｅ ８ (TDAG8) の役割解析 (2016)
  • Author(s): 小野 達貴, 西 俊英, 長坂 明臣, 仲矢 道雄, 岡島 史和, 黒瀬 等
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-03-27
• [Presentation] 心筋梗塞時におけるプロトン感知性受容体（TDAG8）の役割解明 (2015)
  • Author(s): 西俊英、長坂明臣、仲矢道雄、黒瀬等
  • Organizer: 第８８回 日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-03-20