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Study on drug discovery in the central nervous system targeting ERM protein

Research Project

Project/Area Number 26860058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pharmacology in pharmacy
Research InstitutionGifu Pharmaceutical University

Principal Investigator

Inden Masatoshi  岐阜薬科大学, 薬学部, 准教授 (70512424)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsERMタンパク質 / ミクログリア / 炎症性サイトカイン / ERMタンパク / スキャホールドタンパク質 / パーキンソン病 / ドパミン神経 / ドパミン神経細胞
Outline of Final Research Achievements

Moesin (Msn), a member of Ezrin/Radixin/Moesin (ERM) proteins links between membrane proteins and actin cytoskeleton, and contributes to maintenance of cellular function and morphology. In this study, we examined the function of Msn in microglia. We found that Msn was involved in the release mechanism of tumor necrosis factor α in BV2 cells. In addition, Msn interacted with inducible nitric oxide synthase (iNOS) via ERM binding protein 50 (EBP 50). It was also revealed that the phagocytosis ability of BV2 cells knocked down by Msn was decreased due to the abnormal actin skeleton. Furthermore, activation of microglia by administration of LPS was attenuated in Msn knockout mice. These results suggest that it may be the foundation of drug discovery research for microglia targeting Msn.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] Inhibition of monocarboxylate transporter 1 suppresses the proliferation of glioblastoma stem cells.2016

    • Author(s)
      Takada T, Takata K, Ashihara E.
    • Journal Title

      J Physiol Sci

      Volume: 66 Pages: 74-81

    • DOI

      10.1016/j.neuint.2016.02.008

    • NAID

      40020922534

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ezrin mediates neuritogenesis via down-regulation of RhoA activity in cultured cortical neurons2014

    • Author(s)
      Matsumoto Y, Inden M, Tamura A, Hatano R, Tsukita S, Asano S
    • Journal Title

      PLoS One

      Volume: 9 Issue: 8 Pages: e105435-e105435

    • DOI

      10.1371/journal.pone.0105435

    • URL

      https://localhost/en/publications/e9f0f7db-fabb-4d6d-b367-46728c4de2ba

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ミクログリアのiNOS発現におけるERMファミリーの役割2016

    • Author(s)
      阿部 春果、位田 雅俊、二階堂 七海、栗田 尚佳、保住 功
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] VPS35ノックダウン細胞における2価鉄イオンの局在変化2016

    • Author(s)
      内田 泰弘, 位田 雅俊, 名嘉 優熙, 栗田 尚佳, 平山 祐,永澤 秀子, 保住 功
    • Organizer
      第89回薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
  • [Presentation] パーキンソン病モデルラットにおけるガランタミンのニコチン受容体を介した薬物旋回運動2015

    • Author(s)
      位田 雅俊、北村 佳久、下濱俊
    • Organizer
      第127回日本薬理学会近畿部会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report
  • [Presentation] ミクログリアの炎症性サイトカイン放出におけるERMファミリーの役割2015

    • Author(s)
      全並 美穂、位田 雅俊、打越 舞、保住 功
    • Organizer
      第135回日本薬学会年会
    • Place of Presentation
      神戸 兵庫医療大学 他
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 原因遺伝子を軸にした神経変性疾患の創薬研究2014

    • Author(s)
      位田雅俊
    • Organizer
      創薬科学フロンティアシンポジウム
    • Place of Presentation
      京都 京都薬科大学
    • Year and Date
      2014-11-23
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Ezrin regulates neuritogenesis through down-regulation of RhoA/ROCK/MLC2 pathway2014

    • Author(s)
      Yosuke Matsumoto, Masatoshi Inden, Atsushi Tamura, Ryo Hatano, Sachiko Tsukita, Shinji Asano
    • Organizer
      Neuroscience2014
    • Place of Presentation
      Washington DC
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 活性化ミクログリアにおけるERMタンパク質の機能解析2014

    • Author(s)
      打越 舞、位田雅俊、全並美穂、金子雅幸、保住 功
    • Organizer
      次世代を担う創薬・医療薬理シンポジウム2014
    • Place of Presentation
      大阪 近畿大学
    • Year and Date
      2014-08-30
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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