| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The alcoholic extracts of three crude drugs [the roots and rhizomes of Saposhnikovia divaricata (Umbelliferae), the leaves of Thujopsis dolabrata, and the barks of Marsdenia cundurango], three Amaryllidaceae plants (Lycoris radiata, Galanthus elwesii, and Haemanthus multiflorus), a Ranunculaceae plant (Helleborus foetidus), and a Liliaceae plant (Ornithogalum saundersiae), were evaluated for their cytotoxic activity against HL-60 human leukemia cells. As a result of bioassay-guided fractionation of the extracts, a chromon derivative from S. divaricata, a pregnane glycoside from the barks of M. cundurango, a bufadienolide derivative (HF-1) and anemonin from H. foetidus, a cholestane glycoside (OS-1) from O. saundersiae were obtained as active substances, and all of them induced apoptosis in HL-60 cells. HF-1, anemonin, and OS-1 arrested cell cycle progression in G2/M phase. Anemonin showed microtuble assembly in A549 human lung cancer cells by immunofluoresence microscopy.
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