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Individual seed epigenetics profiling for rice to detect unauthorized GM trait

Research Project

Project/Area Number 26860091
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionNational Institute of Health Sciences

Principal Investigator

Nakamura Kosuke  国立医薬品食品衛生研究所, 生化学部, 主任研究官 (60570926)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords作物 / 遺伝子組換え / DNA / 検知法 / メチル化 / 食品 / カルス / 種子 / 遺伝子導入 / ゲノム / DNAメチル化
Outline of Final Research Achievements

Rice callus developed from one grain was cultured under various stress conditions during cellular differentiation. Then, DNA methylation patters in rice endogenous Actin1 promoter (Act1p) were analyzed in wild-type rice and genetically modified (GM) rice that has transgenic Act1p. The DNA methylation pattern of Act1p in the GM rice was found methylated as compared to that in the wild-type rice under low regeneration condition. Also, DNA methylation patterns of Cauliflower Mosaic Virus (CaMV) 35S RNA promoter (P35S) in transgenic construct inserted into GM crop genomes and crop-infected CaMV genomes were analyzed. Multivariable statistical analysis of DNA methylation patterns indicated that the GM crops were grouped different from the crop-infected CaMV. Detection of the DNA methylation patterns in P35S could differentiate the presence of the GM crops for food ingredients and/or the CaMV in food commodities.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (39 results)

All 2016 2015 2014

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (28 results) (of which Int'l Joint Research: 3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK2016

    • Author(s)
      Nakamura, K., Kondo, K., Akiyama, H., Ishigaki, T., Noguchi, A., Katsumata, H., Takasaki, K., Futo, S., Sakata, K., Fukuda, N., Mano, J., Kitta, K., Tanaka, H., Akashi, R., & Nishimaki-Mogami, T.
    • Journal Title

      Data in Brief

      Volume: 7 Pages: 1165-1170

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method2016

    • Author(s)
      Nakamura, K., Kondo, K., Akiyama, H., Ishigaki, T., Noguchi, A., Katsumata, H., Takasaki, K., Futo, S., Sakata, K., Fukuda, N., Mano, J., Kitta, K., Tanaka, H., Akashi, R., Nishimaki-Mogami, T.
    • Journal Title

      Food Chemistry

      Volume: 205 Pages: 272-279

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Oral administration of apple condensed tannins delays rheumatoid arthritis development in mice via down-regulation of T helper 17 (Th17) cell responses2015

    • Author(s)
      Nakamura, K., Matsuoka, H., Nakashima, S., Kanda, T., Nishimaki-Mogami, T., Akiyama, H.
    • Journal Title

      Molecular Nutrition & Food Research

      Volume: 59 Pages: 1406-1410

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the specificity, stability, and PCR efficiency of six rice endogenous sequences for detection analyses of genetically modified rice2015

    • Author(s)
      Takabatake, R., Onishi, M., Futo, S., Minegishi, Y., Noguchi, A., Nakamura, K., Kondo, K., Teshima, R., Mano, J., Kitta, K.
    • Journal Title

      Food Control

      Volume: 50 Pages: 949-955

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel trait-specific real-time PCR method enables quantification of genetically modified (GM) maize content in ground grain samples containing stacked GM maize2015

    • Author(s)
      Noguchi, A., Akiyama, H., Nakamura, K., Sakata, K., Minegishi, Y., Mano, J., Takabatake, R., Futo, S., Kitta, K., Teshima, R., Kondo, K., Nishimaki-Mogami, T.
    • Journal Title

      European Food Research and Technology

      Volume: 240 Pages: 413-422

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a simple detection method for genetically modified papaya PRSV-YK2014

    • Author(s)
      Tanaka, H., Kitazaki, Y., Nakamura, K., Akiyama, H., Akashi, R.
    • Journal Title

      Ikushugaku Kenkyu

      Volume: 55 Pages: 231-246

    • NAID

      130004850640

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Examination on the detection of common DNA sequence of genetically modified tomatoes in processed vegetable foods2014

    • Author(s)
      Kitagawa, M., Nakamura, K., Kondo, K., Ubukata, S., Akiyama, H.
    • Journal Title

      Food Hygiene and Safety Science

      Volume: 55 Pages: 247-253

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of pBT63, a positive control plasmid for qualitative detection of genetically modified rice2014

    • Author(s)
      Minegishi, Y., Mano, J., Takabatake, R., Nakamura, K., Kondo, K., Kato, Y., Kitta, K., Akiyama, H.
    • Journal Title

      Japanese Journal of Food Chemistry and Safety

      Volume: 21 Pages: 48-56

    • NAID

      110009810365

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of direct real-time PCR system applicable to a wide range of food and agricultural products2014

    • Author(s)
      Mano, J., Hatano, S., Futo, S., Minegishi, Y., Ninomiya, K., Nakamura, K., Kondo, K., Teshima, R., Takabatake, R., Kitta, K.
    • Journal Title

      Food Hygiene and Safety Science

      Volume: 55 Pages: 25-33

    • NAID

      130003394385

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] DNA methylation pattern analysis of common plant virus promoter used to develop genetically modified crops2015

    • Author(s)
      Nakamura, K., Ishigaki, T., Hanada, K., Akimoto, S., Kondo, K., Nishimaki-Mogami, T.
    • Organizer
      PacifiChem2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 我が国における未承認遺伝子組換えパパイヤの食品への混入に関する事例と検知法開発の現状2015

    • Author(s)
      中村公亮、近藤一成、穐山浩、石垣拓実、野口秋雄、坂田こずえ、福田のぞみ、大森清美、布施谷実聡、川上浩、田中秀典、明石良、真野潤一、橘田和美、最上(西巻)知子
    • Organizer
      第52回全国衛生化学技術協議会年会
    • Place of Presentation
      静岡
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子組換えトウモロコシの新規スクリーニング検査法の妥当性評価2015

    • Author(s)
      野口秋雄、中村公亮、真野潤一、高畠令王奈、橘田和美、近藤一成、最上(西巻)知子
    • Organizer
      第52回全国衛生化学技術協議会年会
    • Place of Presentation
      静岡
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝毒性試験および全ゲノム解析を用いたCRISPR/Cas9のDNA2本鎖切断ポテンシャル2015

    • Author(s)
      福田(佐藤)のぞみ、近藤一成、坂田こずえ、中村公亮、野口秋雄、最上(西巻)知子
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] A new analytical methodology for unknown genetically modified organisms using linear-amplified mediated PCR (LAM-PCR)2015

    • Author(s)
      Kondo, K., Sakata, K., Noguchi, A., Nakamura, K., Fukuda, N., Ishigaki, T., Nishimaki-Mogami, T.
    • Organizer
      7th International Symposium on Recent Advances in Food Analysis
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-11-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid identification of poisonous Entoloma rhodopolium and edible E. sarcopum mushrooms using PCR-restriction fragment length polymorphism and real-time PCR2015

    • Author(s)
      Kondo, K., Sakata, K., Nakamura, K., Noguchi, A., Fukuda, N., Ishigaki, T., Nishimaki-Mogami, T
    • Organizer
      7th International Symposium on Recent Advances in Food Analysis
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-11-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LAM-PCRを用いた組換え作物中の未知領域解析法の検討2015

    • Author(s)
      坂田こずえ、近藤一成、野口秋雄、中村公亮、福田のぞみ、石垣拓実、最上(西巻)知子
    • Organizer
      第110回 日本食品衛生学会学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子組換えトウモロコシの簡易粒検査法の開発2015

    • Author(s)
      野口秋雄、町井香苗、中村公亮、真野潤一、高畠令王奈、橘田和美、川上浩、近藤一成、最上(西巻)知子
    • Organizer
      第110回 日本食品衛生学会学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] ツキヨタケのPCR-RFLPを用いた迅速同定法の検討(第2法):加熱、消化処理サンプルへの適用2015

    • Author(s)
      菅野陽平、坂田こずえ、野口秋雄、中村公亮、小林友子、福田のぞみ、佐藤正幸、青塚圭二、鈴木智宏、最上知子、手島玲子、近藤一成
    • Organizer
      第110回 日本食品衛生学会学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 安全性未承認遺伝子組換えパパイヤ(PRSV-HN系統)の検出と検知法開発2015

    • Author(s)
      中村公亮、近藤一成、石垣拓実、野口秋雄、坂田こずえ、福田のぞみ、大森清美、真野潤一、橘田和美、最上(西巻)知子
    • Organizer
      第110回 日本食品衛生学会学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 未承認遺伝子組換え食品検知法の開発:未承認遺伝子組換えジャガイモ検知を例に2015

    • Author(s)
      中村公亮、石垣拓実、坂田こずえ、福田のぞみ、野口秋雄、穐山浩、近藤一成、真野潤一、高畠令王奈、橘田和美、最上(西巻)知子
    • Organizer
      第1回 次世代を担う若手のためのレギュラトリーサイエンスフォーラム
    • Place of Presentation
      千葉
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 食品遺伝子検査を簡易化するダイレクトリアルタイムPCR2015

    • Author(s)
      真野潤一、波田野修子、布藤聡、峯岸恭孝、二宮健二、中村公亮、近藤一成、手島玲子、高畠令王奈、橘田和美:食品遺伝子検査を簡易化するダイレクトリアルタイムPCR
    • Organizer
      2015年度AOAC International日本セクション年次大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 次世代ゲノム編集技術による遺伝子組換え食品の内在性遺伝子発現への影響2015

    • Author(s)
      中村公亮、石垣拓実、近藤一成、最上(西巻)知子
    • Organizer
      日本食品化学学会 第21回総会・学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 遺伝子組換えヒヨコマメ検査法確立に向けたヒヨコマメ内在性遺伝子(CaNCED)特異的検知法の開発2015

    • Author(s)
      石垣拓実、中村公亮、近藤一成、最上(西巻)知子
    • Organizer
      日本食品化学学会 第21回総会・学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 標的DNAのメチル化の頻度およびパターン解析による新規GM検知法確立の試み2014

    • Author(s)
      中村公亮、小林友子、近藤一成、最上(西巻)知子
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] CaNCED配列を標的としたヒヨコマメ内在性遺伝子検知法2014

    • Author(s)
      中村公亮、近藤一成、小林友子、野口秋雄、高畠令王奈、橘田和美、最上(西巻)知子
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 加工食品中の遺伝子組換えコメ検出のためのシリカモノリスカラムを用いた新しいDNA抽出精製法の検討2014

    • Author(s)
      東城雄満、西野浩史、中村公亮、近藤一成、深谷崇、大平真義、中西和樹
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 食品中に含有する添加物のDNA精製効率に与える影響について2014

    • Author(s)
      中西希代子、中村公亮、近藤一成、池田惠
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Multiplex real-time PCRを用いたクサウラベニタケとその近縁種の同定2014

    • Author(s)
      坂田こずえ、近藤一成、中村公亮、野口秋雄、小林友子、福田のぞみ、最上(西巻)知子
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子組換えトウモロコシの新規スクリーニング検査法の開発2014

    • Author(s)
      野口秋雄、中村公亮、真野潤一、高畠令王奈、峯岸恭孝、橘田和美、手島玲子、近藤一成、最上(西巻)知子
    • Organizer
      第108回 日本食品衛生学会学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 成長ホルモン遺伝子を組換えた遺伝子組換えサケ検知法の試験室間共同試験による妥当性確認2014

    • Author(s)
      中村公亮、近藤一成、小林友子、坂田こずえ、野口秋雄、名古屋博之、真野潤一、橘田和美、最上(西巻)知子
    • Organizer
      第51回全国衛生化学技術協議会年会
    • Place of Presentation
      大分
    • Year and Date
      2014-11-20 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 2010年度米国産不分別トウモロコシ試料における遺伝子組換えトウモロコシの混入率と系統分析2014

    • Author(s)
      野口秋雄、坂田こずえ、真野潤一、中村公亮、高畠令王奈、峯岸恭孝、橘田和美、穐山浩、手島玲子、近藤一成、最上(西巻)知子
    • Organizer
      第51回全国衛生化学技術協議会年会
    • Place of Presentation
      大分
    • Year and Date
      2014-11-20 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] RFLPおよびReal-time PCR法を用いたクサウラベニタケ複合種の分析法2014

    • Author(s)
      坂田こずえ、近藤一成、中村公亮、野口秋雄、小林友子、福田のぞみ、最上(西巻)知子
    • Organizer
      第51回全国衛生化学技術協議会年会
    • Place of Presentation
      大分
    • Year and Date
      2014-11-20 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] A novel transgenic construct-specific real-time PCR detection method for genetically modified salmon in foods2014

    • Author(s)
      Nakamura, K., Kondo, K., Akiyama, H., Kobayashi, T., Noguchi, A., Nagoya, H., Takabatake, R., Kitta, K., Plouffe, D., Buchanan, J., Nishimaki-Mogami, T.
    • Organizer
      128th AOAC Annual Meeting & Exposition
    • Place of Presentation
      Florida, USA
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子組換えイネ検出のためのイネ種共通内在性配列の検討2014

    • Author(s)
      高畠令王奈、大西真理、布籐聡、峯岸恭孝、野口秋雄、中村公亮、近藤一成、手島玲子、真野潤一、橘田和美
    • Organizer
      2014年度AOAC International日本セクション年次大会
    • Place of Presentation
      東京
    • Year and Date
      2014-06-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 次世代ゲノム編集技術を用いた人工プロモーター挿入によるグロビン遺伝子クラスターループ内の遺伝子発現量の調節2014

    • Author(s)
      中村公亮、小林友子、近藤一成、最上(西巻)知子
    • Organizer
      日本食品化学学会 第20回 総会・学術大会
    • Place of Presentation
      東京
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 未承認遺伝子組換え食品の検知法の開発に関する研究2014

    • Author(s)
      中村公亮
    • Organizer
      日本食品化学学会 第20回 総会・学術大会
    • Place of Presentation
      東京
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] DNAマイクロアレイによる未承認遺伝子組換えパパイヤのスクリーニング検査法2014

    • Author(s)
      伊東 篤志、田口 朋之、田名網 健雄、羽田 聖治、中村 公亮、近藤 一成、穐山 浩、手島 玲子、何 思厳、宮原 平、山田 晃世、小関 良宏
    • Organizer
      日本食品化学学会 第20回 総会・学術大会
    • Place of Presentation
      東京
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 遺伝子組換え植物の判定法2016

    • Inventor(s)
      中村公亮、小林友子、近藤一成
    • Industrial Property Rights Holder
      公益財団法人ヒューマンサイエンス振興財団
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-02-04
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 遺伝子組換え植物の判定方法2014

    • Inventor(s)
      中村公亮、小林友子、近藤一成
    • Industrial Property Rights Holder
      中村公亮、小林友子、近藤一成
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-143773
    • Filing Date
      2014-07-14
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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