• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel gene medicines based on the multifunctional nano-device using the regulatory-approved medical products

Research Project

Project/Area Number 26860107
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionNagasaki University

Principal Investigator

KODAMA Yukinobu  長崎大学, 医歯薬学総合研究科(薬学系), 助教 (50448510)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords遺伝子デリバリー / 医薬品 / 硫酸プロタミン / コンドロイチン硫酸 / siRNA
Outline of Final Research Achievements

In this study, we developed the novel gene vector using regulatory-approved medical products. We used novo-protamine sulfate (PRT), chondroitin sulfate sodium (CS), stronger neo-minophagen C (GL), and sodium hyaluronate (HA) ophthalmic solution as medical products. We successfully prepared the pDNA-PRT complexes (PRT complexes) and pDNA-PRT-CS complexes (CS complexes) by optimization of the mix ratio and preparation process. PRT complexes and CS complexes showed high gene expression. Although PRT complexes showed slight cytotoxicity, CS complexes showed no cytotoxicity. Also, the gene expression of PRT complexes was greatly reduced in the presence of FBS. The presence of FBS, however, did not greatly affect gene expression of the CS complex. CS complexes showed high gene expression in the spleen after intravenous administration.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 5 results) Presentation (14 results)

  • [Journal Article] Application of biodegradable dendrigraft poly-l-lysine to a small interfering RNA delivery system.2017

    • Author(s)
      Kodama Y, Kuramoto H, Mieda Y, Muro T, Nakagawa H, Kurosaki T, Sakaguchi M, Nakamura T, Kitahara T, Sasaki H.
    • Journal Title

      Journal of Drug Targeting

      Volume: 25 Issue: 1 Pages: 49-57

    • DOI

      10.1080/1061186x.2016.1184670

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a Multi-functional Nano-device for Safe and Effective Gene Delivery to Target Organs.2016

    • Author(s)
      Kodama Y.
    • Journal Title

      Yakugaku Zasshi.

      Volume: 136 Pages: 1533-1539

    • NAID

      130005267661

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quaternary complexes modified from pDNA and poly-l-lysine complexes to enhance pH-buffering effect and suppress cytotoxicity.2015

    • Author(s)
      Kodama Y, Yatsugi Y, Kitahara T, Kurosaki T, Egashira K, Nakashima M, Muro T, Nakagawa H, Higuchi N, Nakamura T, Sasaki H.
    • Journal Title

      Journal of Pharmaceutical Sciences

      Volume: 104 Issue: 4 Pages: 1470-1477

    • DOI

      10.1002/jps.24364

    • NAID

      120006987163

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Secure and effective gene delivery system of plasmid DNA coated by polynucleotide.2015

    • Author(s)
      Kodama Y, Ohkubo C, Kurosaki T, Egashira K, Sato K, Fumoto S, Nishida K, Higuchi N, Kitahara T, Nakamura T, Sasaki H.
    • Journal Title

      J Drug Target.

      Volume: 23 Issue: 1 Pages: 43-51

    • DOI

      10.3109/1061186x.2014.950665

    • NAID

      120006986293

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection.2015

    • Author(s)
      Mbanefo EC, Kumagai T, Kodama Y, Kurosaki T, Furushima-Shimogawara R, Cherif MS, Mizukami S, Kikuchi M, Huy NT, Ohta N, Sasaki H, Hirayama K.
    • Journal Title

      Parasitol Int.

      Volume: 64 Issue: 4 Pages: 24-31

    • DOI

      10.1016/j.parint.2015.01.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Splenic Gene Delivery System Using Self-assembling Nano-complex with Phosphatidylserine Analog.2015

    • Author(s)
      Kurosaki T, Nakasone C, Kodama Y, Egashira K, Harasawa H, Muro T, Nakagawa H, Kitahara T, Higuchi N, Nakamura T, Sasaki H.
    • Journal Title

      Biol Pharm Bull.

      Volume: 38 Pages: 23-29

    • NAID

      130004872233

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biodegradable nanoparticles composed of dendrigraft poly-L-lysine for gene delivery.2014

    • Author(s)
      Kodama Y, Nakamura T, Kurosaki T, Egashira K, Mine T, Nakagawa H, Muro T, Kitahara T, Higuchi N, Sasaki H.
    • Journal Title

      Eur J Pharm Biopharm.

      Volume: 87 Issue: 3 Pages: 472-479

    • DOI

      10.1016/j.ejpb.2014.04.013

    • NAID

      120006986334

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Novel siRNA delivery system using a ternary polymer complex with strong silencing effect and no cytotoxicity.2014

    • Author(s)
      Kodama Y, Shiokawa Y, Nakamura T, Kurosaki T, Aki K, Nakagawa H, Muro T, Kitahara T, Higuchi N, Sasaki H.
    • Journal Title

      Biol Pharm Bull.

      Volume: 37 Pages: 1274-1281

    • NAID

      130004677533

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] グリコサミノグリカンで被膜した生体分解型遺伝子ベクターの開発2016

    • Author(s)
      三枝 由香莉、兒玉 幸修、岩永 真理恵、北原 隆志、佐々木 均
    • Organizer
      日本薬剤学会第31年会
    • Place of Presentation
      長良川国際会議場(岐阜県、岐阜市)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 関節リウマチ治療を目的としたメトトレキサートを構成成分とする遺伝子ベクターの開発2016

    • Author(s)
      小林 瑞希、兒玉 幸修、北原 隆志、佐々木 均
    • Organizer
      日本薬剤学会第31年会
    • Place of Presentation
      長良川国際会議場(岐阜県、岐阜市)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] ポリアミノ酸で構築した生体分解型マラリアナノワクチンの開発2016

    • Author(s)
      根岸 智奈美、兒玉 幸修、北原 隆志、佐々木 均
    • Organizer
      日本薬剤学会第31年会
    • Place of Presentation
      長良川国際会議場(岐阜県、岐阜市)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 標的臓器への安全かつ高効率な核酸医薬送達を実現する多機能性ナノデバイスの開発2015

    • Author(s)
      兒玉幸修
    • Organizer
      第32回日本薬学会九州支部大会
    • Place of Presentation
      九州保健福祉大学(宮崎県延岡市)
    • Year and Date
      2015-11-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 生体分解型DNAナノワクチンによる悪性黒色腫の増殖抑制2015

    • Author(s)
      徳永彩子、兒玉幸修、北原隆志、佐々木 均
    • Organizer
      第31回日本DDS学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
  • [Presentation] pH緩衝能の増大と毒性軽減を目的とした四重複合体の開発と有用性評価2015

    • Author(s)
      上田由貴、兒玉幸修、三枝由香莉、北原隆志、佐々木 均
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Dendrigraft poly-L-lysine を基材とした遺伝子ベクターの有用性評価2015

    • Author(s)
      徳永彩子、兒玉幸修、樋口則英、北原隆志、佐々木 均
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] アニオン性デンドリプレックスを用いた遺伝子デリバリーシステムの開発2015

    • Author(s)
      野田 稜、兒玉幸修、黑﨑友亮、中村忠博、北原隆志、佐々木 均
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] アニオン性三重複合体の眼疾患用遺伝子ベクターとしての有用性評価2015

    • Author(s)
      永原忠幸、兒玉幸修、北原隆志、中嶋幹郎、佐々木 均
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 抗原提示細胞標的化ナノ粒子を用いた新規がんワクチンの開発2015

    • Author(s)
      花村啓紀、兒玉幸修、北原隆志、中嶋幹郎、佐々木 均
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Presentation] Dendrigraft poly-L-lysineを構成成分とした生分解型siRNAベクターの構築2014

    • Author(s)
      藏本 悠、兒玉 幸修、北原 隆志、佐々木 均
    • Organizer
      日本薬剤学会第29年会
    • Place of Presentation
      大宮ソニックシティビル(大宮)
    • Year and Date
      2014-05-20 – 2014-05-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 医療用医薬品を用いたGMP基準の新規遺伝子ベクターの開発2014

    • Author(s)
      西垣 和香、兒玉 幸修、北原 隆志、佐々木 均
    • Organizer
      日本薬剤学会第29年会
    • Place of Presentation
      大宮ソニックシティビル(大宮)
    • Year and Date
      2014-05-20 – 2014-05-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Secure and effective gene vector of polyamidoamine dendrimer pharmaceutically modified with anionic polymer2014

    • Author(s)
      Yukinobu Kodama, Yumi Yamashita, Takashi Kitahara, Sasaki Hitoshi
    • Organizer
      5th Pharmaceutical Sciences World Congress
    • Place of Presentation
      Melbourne Convention and Exhibition Centre(メルボルン)
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Nanoparticles electrostatically coated with folic acid for effective and safe gene delivery2014

    • Author(s)
      Hitoshi Sasaki, Yukinobu Kodama, Tamami Morishita, Takashi Kitahara
    • Organizer
      5th Pharmaceutical Sciences World Congress
    • Place of Presentation
      Melbourne Convention and Exhibition Centre(メルボルン)
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi