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The pathological role of an orphan transporter in the regulation of cancer cell characteristics

Research Project

Project/Area Number 26860109
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionKumamoto University

Principal Investigator

ITO SHINGO  熊本大学, その他の研究科, 助教 (20466535)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsオーファントランスポーター / がん / プロテオミクス / 肝臓がん / SWATH / 腫瘍形成 / タンパク質翻訳後修飾 / 細胞増殖
Outline of Final Research Achievements

Low SLC22A18 protein expression has been reported to be associated with a poor survival rate in several cancers. In this study, we investigated the expression and functional role of SLC22A18 in HepG2 hepatoblastoma cells. Western blotting confirmed the protein expression of SLC22A18 in HepG2 cells. The invasiveness of SLC22A18-knockdown HepG2 cells was increased, while proliferation and chemotaxis of SLC22A18-knockdown HepG2 cells were reduced. Quantitative whole proteomic analysis using SWATH technology demonstrates a significant change in the intracellular proteins involved in cell proliferation in the SLC22A18-knockdown HepG2 cells. These findings suggest that SLC22A18 plays a pivotal role in the regulation of hepatocarcinoma cancer cell growth characteristics, by altering the expression of cellular proteins.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2015

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Facilitation of cell proliferation by suppression of SLC22A18 in human breast cancer cells, MCF-72015

    • Author(s)
      Yu Fujino, Shingo Ito, Mio Hirayama, Sumio Ohtsuki
    • Organizer
      nternational Symposium on Chronic Inflammatory Diseases, Kumamoto (ISCIDK2015)
    • Place of Presentation
      熊本、熊本大学薬学部大江キャンパス
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SLC22A18 regulates the proliferation of MCF-7 human breast cancer cells by altering cellular protein expression2015

    • Author(s)
      伊藤慎悟
    • Organizer
      第74回日本癌学会
    • Place of Presentation
      名古屋、名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] オーファントランスポーターSLC22A18のタンパク質翻訳後修飾への関与2015

    • Author(s)
      伊藤慎悟、本多玄太郎、赤澤貴憲、平山未央、寺崎哲也、大槻純男
    • Organizer
      第10回トランスポーター研究会年会(JTRA2015)
    • Place of Presentation
      東京、慶應義塾大学 薬学部 芝共立キャンパス
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] オーファントランスポーターのタンパク質翻訳後修飾への関与2015

    • Author(s)
      本多玄太郎、赤澤貴憲、伊藤慎悟、仲村健司、平山未央、寺崎哲也、大槻純男
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎、長崎ブリックホール
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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