Elucidation of molecular mechanism of SV40 VP1 capsid protein to induce aimed cytotoxic T lymphocyte against viruses and its application for vaccine formulation.
| Project/Area Number |
26860112
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 薬学 / 医療薬剤学 / simian virus 40 / カプシド / cytotoxic T lymphocyte / 免疫応答 / ワクチン / 粘膜免疫 / ポリオーマウイルス / SV40 / ウイルス粒子 / 免疫反応 / 獲得免疫 / 細胞傷害性T細胞 / 粘膜ワクチン / がんワクチン / ウイルス様粒子 / 免疫学 / アジュバント / ドラッグデリバリー / 抗原 / エピトープ |
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| Outline of Final Research Achievements |
We have prepared chimeric simian virus 40 (SV40) VP1 protein harboring a foreign epitope to induce aimed cytotoxic T lymphocyte (CTL). Immunization of the protein successfully induced CTL against the inserted foreign epitope, in which the level of CTL induction was over 50 times higher than that induced by classical immunization method using incomplete freund’s adjuvant. During examining the CTL induction property of SV40 VP1, we found that SV40 VP1 significantly induced immune responses against specific immune cells through a specific receptor expressed on the immune cells. We also found that the level of specific chemotactic cytokine secretions induced by SV40 VP1 was higher than that induced by LPS. In addition, as an application of the property of SV40 VP1 that induces strong immune responses against aimed CTL epitope within the SV40 VP1, we have constructed a vaccine against a refractory virus infection using SV40 VP1.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes2015
Author(s)
M.-A. Kawano, K. Doi, H. Fukuda, Y. Kita, K. Imai, T. Inoue, T. Enomoto, M. Matsui, M. Hatakeyama, Y. Yamaguchi, H. Handa
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Journal Title
Biotechnol. Rep.
Volume: 5
Pages: 105-111
DOI
Related Report
Peer Reviewed / Open Access
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