Elucidation of the 3D structure and morphological diversity of the Golgi apparatus by modern 3D imaging technique
| Project/Area Number |
26860128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Asahikawa Medical College (2015-2016)
Niigata University (2014) |
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Principal Investigator |
Koga Daisuke 旭川医科大学, 医学部, 准教授 (30467071)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ゴルジ装置 / 相関顕微鏡観察法 / Array tomography / 3D再構築法 / 3D再構築 / 連続切片SEM法 / SEM / 相関顕微観察法 |
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| Outline of Final Research Achievements |
The present study developed a novel three-dimensional (3D) imaging technique combining array tomography and osmium impregnation methods. This novel method was applied to analysis of the 3D shape of the entire Golgi apparatus, which exhibits a spatially complex structure. The results clearly demonstrate that complete Golgi apparatus structures are both diverse in shape and cell type-dependent, with each apparatus appearing as a single mass located within a large cytoplasmic region. In addition, morphological changes of the Golgi apparatus were analyzed in gonadotropes before and after castration by correlative light and scanning electron microscopy paired with a combination of immunofluorescence imaging and osmium maceration methods.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Direct evidence for activated CD8+ T cell transmigration across portal vein endothelial cells in liver graft rejection2016
Author(s)
Kariya T, Ueta H, Xu XD, Koga D, Ezaki T, Yu E, Kusumi S, Kitazawa Y, Sawanobori Y, Ushiki T, Issekutz T, Matsuno K
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Journal Title
J Gastroenterol.
Volume: 51
Issue: 10
Pages: 985-998
DOI
Related Report
Peer Reviewed
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[Presentation] SEM試料作製法の基礎2017
Author(s)
甲賀大輔
Organizer
第73回日本顕微鏡学会学術講演会
Place of Presentation
札幌コンベンションセンター
Year and Date
2017-05-30
Related Report
Invited
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