Molecular mechanisms and roles of mitochondrial dynamics in development and diseases of myelinated axons
| Project/Area Number |
26860129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | University of Yamanashi |
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Principal Investigator |
OHNO Nobuhiko 山梨大学, 総合研究部, 准教授 (10432155)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Mitochondria / Myelination / Demyelination / Electron microscopy / Conductive resin / 軸索 / ミトコンドリア / 脱髄 / 髄鞘 |
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| Outline of Final Research Achievements |
We demonstrated alterations of mitochondrial distribution and dynamics during development of mouse central nervous system. In addition, changes of mitochondrial dynamics were caused specifically in demyelinated segments in the mouse dysmyelination model. In the 3D ultrastructural analyses using scanning electron microscopy, improvement of tissue preparation procedures and development of conductive resins were explored to achieve patent application. The carbon based conductive resin could reduce charging and improve imaging in the 3D ultrastructural analyses. The obtained results were published as several papers and presented in conferences.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy2016
Author(s)
Thai TQ, Nguyen HB, Saitoh S, Wu B, Saitoh Y, Shimo S, Elewa YH, Ichii O, Kon Y, Takaki T, Joh K, Ohno N.
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Journal Title
Med Mol Morphol
Volume: Epub ahead of print
Issue: 3
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Differential roles of microglia and monocytes in the inflamed central nervous system2014
Author(s)
Ryo Yamasaki, Haiyan Lu, Oleg Butovsky, Nobuhiko Ohno, Anna M. Rietsch, Ron Cialic, Pauline M. Wu, Camille E. Doykan, Jessica Lin, Anne C. Cotleur, Grahame Kidd, Musab M. Zorlu, Nathan Sun, Weiwei Hu, LiPing Liu, Jar-Chi Lee, Sarah E. Taylor, Lindsey Uehlein, Debra Dixon, Jinyu Gu, Crina M. Floruta, Min Zhu, et al.
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Journal Title
J Exp Med.
Volume: 211
Issue: 8
Pages: 1533-1549
DOI
Related Report
Peer Reviewed
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