Visualization of the phosphoinositide localization during endocytosis
| Project/Area Number |
26860132
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Takatori Sho 東京大学, 薬学研究科(研究院), 助教 (80624361)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ホスホイノシチド / エンドサイトーシス / 急速凍結法 / 凍結割断レプリカ |
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| Outline of Final Research Achievements |
The spatio-temporal regulation of phosphoinositide generation is critical for endocytosis. However, the precise localization of individual phosphoinositide remains unclear, due to the technical difficulty in visualizing membrane lipids. In the present study, the quick-freezing and freeze-fracture replica labeling method was applied to reveal the distribution of phosphoinositides during endocytosis. The specific labeling of PI(3,4)P2 and PI(3,5)P2 were established. Notably, PI(3,5)P2 was found to be heterogeneously distributed in the tubular endosome.
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Report
(3 results)
Research Products
(9 results)
