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Elusiodation of novel spatiotemporal mechanisms of synapse elimination by groups of complement proteins

Research Project

Project/Area Number 26860148
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General physiology
Research InstitutionKeio University

Principal Investigator

Suzuki Kunimichi  慶應義塾大学, 医学部, 訪問研究員 (10713703)

Research Collaborator Aricescu A. Radu  University of Oxford, Welcome Trust Centre for Human Genetics Division of Structural Biology, Professor
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords補体 / シナプス / C1q / C3 / シナプス除去 / AMPA受容体
Outline of Final Research Achievements

It has been suggested so far that classical complement pathway initiated by complement C1q is associated to synapse elimination, but spatio-temporal mechanism how to determine whether the synapse should be pruned or not is still unclear. This research focused not only on C1q but also on the downstream of classical complement signaling. Reconstitution of C1 complex by C1q, C1r and C1s showed the binding on neurons but the binding pattern was similar to the binding of C1q on neurons, suggesting that C1q was enough to bind on neurons. Interestingly, the labeling of C1q was well colocalized with the signals detected by antibody against specific pattern of sugar chains, and was abolished by the enzyme which degraded the sugar chains. As the other topic, in order to develop novel molecules to control synapse formation, artificial excitatory synapse connector was elaborately analyzed and synapse disconnectors were also designed and analyzed about the feature such as binding specificity.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] University of Oxford/Wellcome Trust Centre for Human Genetics/Division of Structural Biology(United Kingdom)

    • Related Report
      2015 Annual Research Report
  • [Presentation] Does complement C1q regulate synapse pruning via binding to chondroitin sulfate proteoglycan2016

    • Author(s)
      Kunimichi Suzuki, Wataru Kakegawa, Eriko Miura, Michisuke Yuzaki
    • Organizer
      The 3rd International Symposium on Glyco-Neuroscience
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-01-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規の人工的シナプス接続分子、Cerebellin-Pentraxin(CPTX)を用いたシナプス形成および可塑性の制御2015

    • Author(s)
      鈴木邦道, Jonathan Elegheert, Amber Clayton, A. Radu Aricescu, 柚崎通介
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場・国際展示場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] cerebellin 1 におけるneurexin結合ドメインの解析~人為的なシナプス解除法の開発を目指して2015

    • Author(s)
      岩本創哉, 鈴木邦道, 柚崎通介
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場・国際展示場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation into molecular mechanism of synapse elimination mediated by complement C1q and C32014

    • Author(s)
      Kunimichi Suzuki, Michisuke Yuzaki
    • Organizer
      2014 Joint Meeting of the Society for Glycobiology and the Japanese Society of Carbohydrate Research
    • Place of Presentation
      Honolulu, Hawaii,USA
    • Year and Date
      2014-11-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Investigation into molecular mechanism of synapse elimination mediated by complement C1q and C32014

    • Author(s)
      Kunimichi Suzuki, Michisuke Yuzaki
    • Organizer
      第8回領域班会議“Glycans in Neuroscience” (Satellite Symposium II)
    • Place of Presentation
      Honolulu, Hawaii,USA
    • Year and Date
      2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Modulation of synapse formation and plasticity by a novel artificial synapse connector, Cerebellin-Pentraxin (CPTX)2014

    • Author(s)
      Kunimichi Suzuki, Jonathan Elegheert, Amber Clayton, A. Radu Aricescu, Michisuke Yuzaki
    • Organizer
      Fourth Annual Conference of COST Action ECMNET
    • Place of Presentation
      ANTALYA, TURKEY
    • Year and Date
      2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 補体C1q, C3は正常シナプスを除去するのか?-分子機構の探索に向けて2014

    • Author(s)
      鈴木邦道、柚崎通介
    • Organizer
      第7回神経糖鎖生物学領域班会議
    • Place of Presentation
      ヤマハリゾートつま恋、静岡
    • Year and Date
      2014-05-25
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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