Relationship between myosin heavy chain expression and muscle force following anterior cruciate ligament reconstruction

• Project/Area Number: 26860151
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Kobayashi Tetsuo 聖マリアンナ医科大学, 医学部, 講師 (20449383)
• Research Collaborator: OGURA Yuji ; KUROSAKA Mitsutoshi ; SUZUKI Tomohiro ; GOTO Katsumasa ; MINAKAWA Naoki ; KISHIRO Satoshi ; UEHARA Kenji ; BEPPU Moroe ; NIKI Hisateru
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 筋力回復 / 膝前十字靱帯 / ミオシン重鎖 / リハビリテーション / 膝前十字靭帯断裂 / スポーツ復帰 / 骨格筋筋線維タイプ / 前十字靭帯再建術 / 骨格筋 / 骨格筋線維タイプ / 前十字靭帯断裂

Outline of Final Research Achievements

Anterior cruciate ligament (ACL) injury and reconstruction are limiting factors to join sport activity. This study was focus on the muscle force to return sport activity after ACL reconstruction. The purpose of this study was to investigate the relationship between myosin heavy chain expression and the recovery of leg extension force following ACL reconstruction. We hypothesized that the recovery of knee function post ACL reconstruction is closely associated with muscle fiber type.To investigate fiber type in skeletal muscle, a small muscle sample was dissected from semitendinosus muscle of each patient in the operation of ACL reconstruction. Myosin heavy chain (MyHC) composition in muscle sample was evaluated by SDS-PAGE.
The present study shows the significant positive relationship between the recovery rate of leg extension force and the composition of type IIa MyHC isoform 12 months after ACL reconstruction.

Research Products

• [Presentation] Relationship between myosin heavy chain expression and leg extension force following anterior cruciate ligament reconstruction (2017)
  • Author(s): Tetsuo Kobayashi
  • Organizer: ORS 2017
  • Place of Presentation: San Diego/ USA
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] 膝前十字靱帯再建術後の脚伸展筋力と骨格筋筋線維タイプの関係 (2017)
  • Author(s): 小林哲士
  • Organizer: 第90回 日本整形外科学会学術集会
• [Presentation] Relationship between myosin heavy chain expression and leg extension force following anterior cruciate ligament reconstruction (2017)
  • Author(s): 小林哲士
  • Organizer: Orthopaedic Research Society 2017
  • Int'l Joint Research
• [Presentation] 膝前十字靱帯断裂症例における骨格筋ミオシン重鎖発現と膝伸展力の関係 (2017)
  • Author(s): 小林哲士
  • Organizer: 第32回 日本整形外科学会基礎学術集会
• [Presentation] 骨格筋筋線維タイプの比率と膝前十字靭帯再建術後の脚伸展筋力回復の関係 (2016)
  • Author(s): 小林哲士
  • Organizer: 第31回 日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡国際会議場（福岡県博多区）
  • Year and Date: 2016-10-13
• [Presentation] 骨格筋筋線維タイプの比率と膝前十字靭帯再建術後の筋力回復の関係について (2016)
  • Author(s): 小林哲士
  • Organizer: 第8回 日本関節鏡・膝・スポーツ整形外科学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-07-29
• [Presentation] 骨格筋筋線維タイプの比率と膝前十字靭帯再建術後の筋力回復の関係について (2016)
  • Author(s): 小林哲士
  • Organizer: 第8回 JOSKAS
  • Place of Presentation: 福岡国際会議場（福岡県博多区）
  • Year and Date: 2016-07-28
• [Presentation] 膝前十字靭帯再建術後の骨格筋筋線維タイプ別の筋力回復に関する研究 (2016)
  • Author(s): 小林哲士
  • Organizer: 第53回日本リハビリテーション医学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-06-10
• [Presentation] 膝前十字靭帯再建術後の骨格筋筋線維タイプ別の筋力回復に関する研究 -第1報- (2016)
  • Author(s): 小林哲士
  • Organizer: 第53回 日本リハビリテーション医学会学術集会
  • Place of Presentation: 京都国際会館（京都府京都市）
  • Year and Date: 2016-06-09
• [Book] 治す！ 山の膝痛 膝の不安を解消する7つの知恵 (2017)
  • Author(s): 小林哲士
  • Total Pages: 208
  • Publisher: 山と渓谷社
  • ISBN: 9784635150323