A physiological study of KCNQ channel functions in the mammalian cerebellum.

• Project/Area Number: 26860154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Irie Tomohiko 国立医薬品食品衛生研究所, 薬理部, 主任研究官 (20546551)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: カリウムチャネル / 活動電位 / 神経細胞 / イオンチャネル

Outline of Final Research Achievements

Voltage gated potassium channels play crucial roles in excitable cells. KCNQ channels belong to the potassium channels and are expressed in the central nervous systems. However, little is known about the physiological roles. Electrophysiological recordings were done from cerebellar acute brain slice preparations. Application of a KCNQ channel blocker or opener reveals that KCNQ channels play roles in maintaining the membrane potential and neuronal excitabilities of cerebellar neurons, suggesting KCNQ channels contribute to the function of the motor coordination conveyed by the cerebellum.

Research Products

• [Journal Article] Effects of 13 developmentally toxic chemicals on the migration of rat cephalic neural crest cells in vitro. (2016)
  • Author(s): Usami M, Mitsunaga K, Miyajima A, Takamatu M, Kazama S, Irie T, Doi O, Takizawa T
  • Journal Title: Congenit Anom (Kyoto). Volume: 56(2) Issue: 2 Pages: 52-59
  • DOI: 10.1111/cga.12121
  • NAID: 130008079222
  • Peer Reviewed / Open Access
• [Journal Article] A synthetic cannabinoid MAM-2201 potently suppresses synaptic transmission via activation of presynaptic CB1 receptors and reduces synaptically-evoked Intracellular Ca2+ transients in cerebellar Purkinje cells. (2015)
  • Author(s): Irie T, Kikura-Hanajiri R, Usami M, Uchiyama N, Goda Y, Sekino Y.
  • Journal Title: Neuropharmacology Volume: xxx Pages: 1-13
  • DOI: 10.1016/j.neuropharm.2015.02.025
  • Peer Reviewed
• [Journal Article] Simple in vitro migration assay for neural crest cells and the opposite effects of all-trans-retinoic acid on cephalic- and trunk-derived cells. (2014)
  • Author(s): Usami M, Mitsunaga K, Irie T, Miyajima A, Doi O.
  • Journal Title: Congenit Anom Volume: 54 Issue: 3 Pages: 184-188
  • DOI: 10.1111/cga.12059
  • Peer Reviewed
• [Journal Article] Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos. (2014)
  • Author(s): Usami M, Mitsunaga K, Irie T, Miyajima A, Doi O.
  • Journal Title: J Toxicol Sci Volume: 39 Pages: 285-292
  • NAID: 130004903990
  • Peer Reviewed / Open Access
• [Presentation] 新規違法ドラッグMAM-2201はCB1受容体を介して神経伝達を強力に抑制し，複雑スパイク誘発性の細胞内Ca2+上昇を減弱させる (2014)
  • Author(s): 入江 智彦, 花尻（木倉） 瑠理, 宇佐見 誠, 内山 奈穂子, 合田 幸広, 関野 祐子
  • Organizer: 生理研研究会 「シナプス・神経ネットワークの機能ダイナミクス」
  • Place of Presentation: 生理学研究所（愛知県）
  • Year and Date: 2014-12-02 – 2014-12-03
• [Presentation] 新規違法ドラッグMAM-2201はCB1受容体を介して神経伝達を強力に抑制し，複雑スパイク誘発性の細胞内Ca2+上昇を減弱させる (2014)
  • Author(s): 入江 智彦, 花尻（木倉） 瑠理, 宇佐見 誠, 内山 奈穂子, 合田 幸広, 関野 祐子
  • Organizer: Neuro2014
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 培養ラット胚におけるバルプロ酸による発生毒性のプロテオミクス解析 (2014)
  • Author(s): 宇佐見 誠，高松 美奈，風間 崇吾，満長 克祥，入江 智彦，宮島 敦子，土井 守，滝沢 達
  • Organizer: 第54回日本先天異常学会学術集会
  • Place of Presentation: 麻布大学（神奈川県）
  • Year and Date: 2014-07-26 – 2014-07-27