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Pharmacogenetic analysis of orexin neuronal projections

Research Project

Project/Area Number 26860157
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental physiology(including physical medicine and nutritional physiology)
Research InstitutionJichi Medical University (2015)
Nagoya University (2014)

Principal Investigator

Inutsuka Ayumu  自治医科大学, 医学部, 助教 (30584776)

Co-Investigator(Renkei-kenkyūsha) YAMANAKA Akihiro  名古屋大学, 環境医学研究所, 教授 (60323292)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsDREADD / オレキシン神経 / 視床下部 / 投射経路 / オレキシン / 薬理遺伝学 / 摂食 / 代謝
Outline of Final Research Achievements

Using orexin-Cre mice and Cre-dependent AAV vectors, we achieved pharmacogenetic activation of orexin neurons. We also achieved selective ablation of orexin neurons using diphtheria toxin fragment A (DTA). Our comprehensive analysis of these transgenic mice revealed a concurrent regulation of sleep/wakefulness, feeding, and metabolism by orexin neurons. In order to manipulate specific orexin neuronal projections, we developed genetic tools for retrograde transport of Cre recombinase.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Insular neural system controls decision-making in healthy and methamphetamine-treated rats2015

    • Author(s)
      Mizoguchi H, Katahira K, Inutsuka A, Fukumoto K, Nakamura A, Wang T, Nagai T, Sato J, Sawada M, Ohira H,Yamanaka A, Yamada K
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 112 (29) Issue: 29 Pages: 3930-3939

    • DOI

      10.1073/pnas.1418014112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A top-down cortical circuit for accurate sensory perception2015

    • Author(s)
      Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
    • Journal Title

      Neuron

      Volume: 86(5) Issue: 5 Pages: 1304-16

    • DOI

      10.1016/j.neuron.2015.05.006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Atomistic design of microbial opsin-based blue-shifted optogenetics tools2015

    • Author(s)
      Kato HE, Kamiya M, Sugo S, Ito J, Taniguchi R, Orito A, Hirata K, Inutsuka A,Yamanaka A, Maturana AD, Ishitani R, Sudo Y, Hayashi S, Nureki O
    • Journal Title

      Nat Commun

      Volume: 6 Issue: 1 Pages: 7177-7177

    • DOI

      10.1038/ncomms8177

    • NAID

      120005745621

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.2014

    • Author(s)
      Tsunematsu T, Ueno T, Tabuchi S, Inutsuka A, Tanaka KF, Hasuwa H, Kilduff TS, Terao A, Yamanaka A.
    • Journal Title

      Journal of Neuroscience

      Volume: 34 Issue: 20 Pages: 6896-6909

    • DOI

      10.1523/jneurosci.5344-13.2014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Concurrent and robust regulation of feeding behavions and metabolism by orexin neurons.2014

    • Author(s)
      Inutsuka A, Inui A, Tabuchi S, Tsunematsu T, Lazarus M.
    • Journal Title

      Neuropharmacology

      Volume: 85 Pages: 451-460

    • DOI

      10.1016/j.neuropharm.2014.06.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vangl2 regulates E-cadherin in epithelial cells.2014

    • Author(s)
      Nagaoka, T., Inutsuka, A., Begum, K., Bin hafiz, K. & Kishi, M.
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 6940-6940

    • DOI

      10.1038/srep06940

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Correlative activity and physiological role of orexin neurons in nociception2015

    • Author(s)
      犬束歩、山下哲、田口徹、チョドリスリカンタ、山中章弘
    • Organizer
      包括型脳科学研究推進支援ネットワーク 平成27 年度 冬のシンポジウム
    • Place of Presentation
      東京都・千代田区
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] The integrative role of orexin neurons in nociception and analgesia2015

    • Author(s)
      Ayumu Inutsuka, Akira Yamashita, Srikanta Chowdhury, Junichi Nakai, Masamichi Okura, Toru Taguchi, Akihiro Yamanaka
    • Organizer
      THE 12TH NIKKO INTERNATIONAL SYMPOSIUM 2015 FRONTIERS IN TRANSLATIONAL NEUROSCIENCE
    • Place of Presentation
      栃木県・下野市
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オレキシン神経は痛み刺激に応じて活動し、侵害受容を調節する2015

    • Author(s)
      犬束歩、山下哲、田口徹、チョドリスリカンタ、山中章弘
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      兵庫県・神戸市
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] The physiological role of orexin neurons in feeding and metabolism revealed by pharmacogenetic activation and chronic ablation2014

    • Author(s)
      Inutsuka A, Inui A, Tabuchi S, Lazarus M, Yamanaka A.
    • Organizer
      Neuroscience 2014 Annual Meeting
    • Place of Presentation
      Washington, D.C.
    • Year and Date
      2014-11-19
    • Related Report
      2014 Research-status Report
  • [Presentation] オレキシン神経特異的な活動操作・細胞死誘導による、摂食・代謝調節機構の解析2014

    • Author(s)
      犬束 歩、乾 あずさ、田淵 紗和子、常松 友美、Michael Lazarus、山中 章弘
    • Organizer
      第61回中部日本生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 薬理遺伝学的手法を用いたオレキシン神経の機能解析2014

    • Author(s)
      犬束 歩
    • Organizer
      平成26年度生理学研究所研究会 グリア細胞機能から迫る脳機能解明
    • Place of Presentation
      岡崎
    • Year and Date
      2014-10-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Pharmacogenetic analysis of concurrent regulation of feeding and metabolism by orexin neurons2014

    • Author(s)
      Inutsuka A, Inui A, Tabuchi S, Lazarus M, Yamanaka A.
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Book] 分子脳科学: 分子から脳機能と心に迫る2015

    • Author(s)
      三品 昌美
    • Total Pages
      291
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report
  • [Book] プログレッシブ生命科学 (第7章 2.時間単位のリズム)2014

    • Author(s)
      犬束歩、山中章弘
    • Publisher
      南山堂
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 投射経路選択的な遺伝子発現制御2014

    • Inventor(s)
      山中章弘、犬束歩
    • Industrial Property Rights Holder
      山中章弘、犬束歩
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-04-30
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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