Search of the novel pineal humoral factor in a mammalian circadian system

• Project/Area Number: 26860163
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Kinki University
• Principal Investigator: IKEGAMI Keisuke 近畿大学, 医学部, 助教 (10709330)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 松果体 / 視交叉上核 / 概日時計 / メラトニン / 時計遺伝子 / 時差ぼけ / 概日リズム

Outline of Final Research Achievements

The functions of humoral factors except melatonin in the pineal body on the light entrainment of the circadian rhythm have not been fully investigated. In melatonin-proficient CBA/N or melatonin-deficient C57BL/6 mice, pinealectomy induced faster resetting for jet-lag. Administration of melatonin to the pinealectomized CBA/N mice did not modify the recovery from the jet lag. 5-HT showed apparent effects on the light resetting process. 5-HT delayed the resetting of locomotor activity rhythm after an abrupt shift of LD cycle, and attenuated the pinealectomy-enhanced phase shift of the circadian locomotor activity as well as c-Fos induction in the SCN after a light pulse during the night. In contrast, 5-HT synthesis inhibitor generated increase of the phase shift as well as increase in c-Fos induction by light. The findings suggest that pineal 5-HT suppresses light entrainment by attenuating the light signal transduction to the central circadian clock in the SCN.

Research Products

• [Journal Article] Tissue-specific post-translational modification allows functional targeting of thyrotropin (2014)
  • Author(s): Ikegami K, Liao XH, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T
  • Journal Title: Cell Reports Volume: 9 Issue: 3 Pages: 801-809
  • DOI: 10.1016/j.celrep.2014.10.006
  • Peer Reviewed / Open Access
• [Journal Article] Thyrotropin receptor knockout changes monoaminergic neuronal system and produces methylphenidate-sensitive emotional and cognitive dysfunction (2014)
  • Author(s): Mouri A, Hoshino Y, Narusawa S, Ikegami K, Mizoguchi H, Murata Y, Yoshimura T, Nabeshima T
  • Journal Title: Psychoneuroendocrinology Volume: 48 Pages: 147-161
  • DOI: 10.1016/j.psyneuen.2014.05.021
  • Peer Reviewed
• [Journal Article] Sperm activation by heat shock protein 70 supports migration of sperm released from sperm storage tubules in Japanese quail (Coturnix japonica) (2014)
  • Author(s): Hiyama G, Matsuzaki M, MizushimaS, Dohra H, Ikegamim K, Yoshimura T, Shiba K, Inaba K, Sasanami T
  • Journal Title: Reproduction Volume: Vol.471 Issue: 2 Pages: 167-178
  • DOI: 10.1530/rep-13-0439
  • Peer Reviewed
• [Journal Article] Low temperature-induced circulating triiodothyronine accelerates seasonal testicular regression. (2014)
  • Author(s): K. Ikegami, Y. Atsumi, E. Yorinaga, H. Ono, I. Murayama1, Y. Nakane, W. Ota, N. Arai, A. Tega, M. Iigo, V. M. Darras, K. Tsutsui, Y. Hayashi, S. Yoshida and T. Yoshimura
  • Journal Title: Endocrinology Volume: 56 Issue: 2 Pages: 647-659
  • DOI: 10.1210/en.2014-1741
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Pineal serotonin modulates entrainment of central circadian clock by light (2016)
  • Author(s): Keisuke Ikegami, Mitsugu Sujino, Mamoru Nagano, Yasufumi Shigeyoshi
  • Organizer: 2016 Society for Research on Biological Rhythms Meeting
  • Place of Presentation: Palm Harbor, Florida, USA
  • Year and Date: 2016-05-21
  • Int'l Joint Research
• [Presentation] Functional analysis of pineal serotonin in mice (2016)
  • Author(s): Keisuke Ikegami, Mitsugu Sujino, Mamoru Nagano, Yasufumi Shigeyoshi
  • Organizer: The 64th NIBB Conference Evolution of Seasonal Timers
  • Place of Presentation: 基礎生物学研究所(岡崎)
  • Year and Date: 2016-04-22
  • Int'l Joint Research
• [Presentation] Functional analysis of pineal serotonin in mice (2015)
  • Author(s): 池上啓介、重吉康史
  • Organizer: 日本時間生物学会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2015-11-21
• [Presentation] Analysis of diurnal yawning rhythm in Wister rat (2015)
  • Author(s): 池上啓介、重吉康史
  • Organizer: 日本生理学会・日本解剖学会
  • Place of Presentation: 神戸（国際会議場）
  • Year and Date: 2015-03-23
• [Presentation] 春告げホルモンTSHにおける組織特異的翻訳後修飾の生理機能 (2014)
  • Author(s): 池上啓介、吉村崇
  • Organizer: 細胞センサーの分子機構・相互関連・ネットワーク研究会
  • Place of Presentation: 岡崎（生理学研究所）
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] 春告げホルモンTSHにおける組織特異的翻訳後修飾の機能解析 (2014)
  • Author(s): Keisuke Ikegami, Xiao-Hui Liao, Yuta Hoshino, Hiroko Ono, Wataru Ota, Yuka Ito, Taeko Nishiwaki-Ohkawa, Chihiro Sato, Ken Kitajima, Masayuki Iigo, Yasufumi Shigeyoshi, Masanobu Yamada, Yoshiharu Murata, Samuel Refetoff, and Takashi Yoshimura
  • Organizer: 日本時間生物学会
  • Place of Presentation: 福岡（九州大学医学部百年講堂）
  • Year and Date: 2014-11-08
• [Presentation] Functional analysis of tissue-specific post-translational modification of springtime hormone TSH (2014)
  • Author(s): Keisuke Ikegami, Xiao-Hui Liao, Yuta Hoshino, Hiroko Ono, Wataru Ota, Yuka Ito, Taeko Nishiwaki-Ohkawa, Chihiro Sato, Ken Kitajima, Masayuki Iigo, Yasufumi Shigeyoshi, Masanobu Yamada, Yoshiharu Murata, Samuel Refetoff, and Takashi Yoshimura
  • Organizer: International Symposium by JSC
  • Place of Presentation: 福岡（九州大学医学部百年講堂）
  • Year and Date: 2014-11-07
• [Presentation] 脊椎動物における光周性の制御機構（Photoperiodic response system in vertebrates） (2014)
  • Author(s): 池上啓介、吉村崇
  • Organizer: 日本動物学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-14
  • Invited
• [Presentation] Analysis of pineal function in melatonin-proficient CBA/N mice (2014)
  • Author(s): Ikegami K, Shigeyoshi Y
  • Organizer: The 30th Anniversary Meeting of Sapporo Symposium on Biological Rhythm
  • Place of Presentation: 札幌（北海道大学）
  • Year and Date: 2014-07-26
• [Presentation] Functional analysis of pineal gland in melatonin-proficient CBA/N mice (2014)
  • Author(s): Ikegami K, Shigeyoshi Y
  • Organizer: 2014 SRBR
  • Place of Presentation: Montana, USA
  • Year and Date: 2014-06-17
• [Presentation] Strain-dependent embryonic lethality induced by flanking genes in homozygous ST8sia2-deficient mice (2014)
  • Author(s): Keisuke Ikegami, Kazumasa Saigoh, Mamoru Nagano, Susumu Kusunoki, Yasufumi Shigeyoshi
  • Organizer: 日本発生学会
  • Place of Presentation: 名古屋（ウィンク愛知）
  • Year and Date: 2014-05-27 – 2014-05-28
• [Remarks] 「春よ来い」生物の巧みな生存戦略、仕組み解明へ 【WPI-ITbM】
  • URL: http://www.aip.nagoya-u.ac.jp/public/nu_research_ja/highlights/detail/0001858.html
• [Remarks] プレスリリース「ホルモンが一人二役を演じる仕組みを解明」
  • URL: http://www.itbm.nagoya-u.ac.jp/ja/research/20141030_Yoshimura_TSH_JP.pdf