| Project/Area Number |
26860164
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 慢性疼痛 / 最初期遺伝子 / 視床下部 / 遺伝子改変動物 / 炎症 / 疼痛 / c-fos / トランスジェニックラット / 脊髄後角 / 抑制性介在ニューロン / 疼痛ストレス |
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| Outline of Final Research Achievements |
We evaluated whether a c-fos-enhanced green fluorescent protein (eGFP) transgenic rat line can be useful for the study of nociceptive pathways and processing. A comparison of eGFP in transgenic rats and Fos-like immunoreactivity (LI) in wild type rats was made in the dorsal spinal cord, paraventricular nucleus (PVN) and supraoptic nucleus (SON). Oxytocin-LI (OXT-LI) was carried out to examine the activation of OXT neurons in the PVN and SON. Following acute nociceptive stimuli, eGFP was maximally expressed at 6 h in the spinal cord and 3 h in the PVN and SON, whereas, Fos-LI was maximally expressed at 1.5 h in all the regions we analyzed. Induction of eGFP in the OXT neurons was more sensitive than that of Fos after acute nociceptive stimuli. These results suggested that the c-fos-eGFP transgenic rat line can be useful for the study of nociceptive pathways and processing.
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