Analysis of molecular mechanisms for nuclear redox maintenance system to support epigenome regulation
| Project/Area Number |
26860180
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | NRF2 / FDH / グルタチオン / ヒストン脱メチル化 / ホルムアルデヒド / GSH / 酸化ストレス / ストレス防御 |
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| Outline of Final Research Achievements |
We analyzed molecular mechanisms of maintenance system for nuclear redox balance to support the epigenome regulation. Using gene-deleted cells and gene-knockout mice, we successfully showed that NRF2 and FDH cooperatively function through the regulation of GSH in the protection from oxidative damage in liver. We also found that formaldehyde increased in the Nrf2::Fdh double mutant cells, compared to respective single mutant cells. To examine the importance of NRF2-FDH-GSH system in cancer cells, we successfully established a model cancer cell line, which rigorously form tumors in allograft experiments in NRF2-dependent manner.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Activation of the NRF2 pathway and its impact on the prognosis of anaplastic glioma patients.2015
Author(s)
Kanamori M*, Higa T, Sonoda Y, Murakami S, Dodo M, Kitamura H, Taguchi K, Shibata T, Watanabe M, Suzuki H, Shibahara I, Saito R, Yamashita Y, Kumabe T, Yamamoto M, Motohashi H*, and Tominaga T.
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Journal Title
Neuro-Oncology
Volume: 17
Issue: 4
Pages: 555-565
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] IDH遺伝子変異がもたらすNRF2機能への影響の解析2014
Author(s)
北村大志, 百々美奈, 比嘉剛志, 金森政之, 本橋ほづみ
Organizer
生命素子による転写環境とエネルギー代謝のクロストーク制御「転写代謝システム」ー平成26年度領域班会議ー
Place of Presentation
仙台 秋保温泉 岩沼屋
Year and Date
2014-07-11 – 2014-07-13
Related Report
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