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Characterization of the novel lipid transporter, Sec14L3

Research Project

Project/Area Number 26860202
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionNational Center for Global Health and Medicine

Principal Investigator

HISHIKAWA Daisuke  国立研究開発法人国立国際医療研究センター, その他部局等, その他 (10569966)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脂質輸送タンパク質 / 肺サーファクタント
Outline of Final Research Achievements

Although pulmonary surfactant is implicated in the various lung functions, molecular mechanisms how the amount of pulmonary surfactant in alveoli is maintained and regulated are still unclear. The aim of this study is to clarify the molecular mechanisms of pulmonary surfactant phospholipid trafficking, secretion, and recycling. To uncover these mechanisms, I focused on the Sec14L3 and Sec14L4, which is the function unknown lipid transporters, highly expressed in the lung.
In this study, I used Sec14L3 and Sec14L4 double knockout (dKO) mice to characterize the functions of these genes in vivo. There are no significant difference in the surfactant phospholipid amount and compositions between control and dKO mice, suggest that they are not critical for the basal lung function. However, I also found that the mRNA expressions of several cytokines were increased in dKO mice than that of control mice after intratracheal administration of lipopolysaccharide.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport.2015

    • Author(s)
      Hashidate-Yoshida T, Harayama T, Hishikawa D, Morimoto R, Hamano F, Tokuoka SM, Eto M, Tamura-Nakano M, Yanobu-Takanashi R, Mukumoto Y, Kiyonari H, Okamura T, Kita Y, Shindou H, Shimizu T.
    • Journal Title

      Elife

      Volume: Apr 21;4 Pages: 4-4

    • DOI

      10.7554/elife.06328

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diversity and function of membrane glycerophospholipids generated by the remodeling pathway in mammalian cells.2014

    • Author(s)
      Hishikawa, D., Hashidate, T., Shimizu, T., and Shindou, H.
    • Journal Title

      J. Lipid Res.

      Volume: 55 Issue: 5 Pages: 799-807

    • DOI

      10.1194/jlr.r046094

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 新規肺胞腔内分泌タンパク質Sec14-like 3の生化学的機能解析2014

    • Author(s)
      菱川大介, 進藤英雄, 原山武士, 小笠原理恵, 諏訪部章, 清水孝雄
    • Journal Title

      分子呼吸器病

      Volume: 19(1) Pages: 122-125

    • Related Report
      2014 Research-status Report
  • [Journal Article] Lysophospholipid acyltransferases mediate phosphatidylcholine diversification to achieve the physical properties required in vivo2014

    • Author(s)
      Takeshi Harayama, Miki Eto, Hideo Shindou, Yoshihiro Kita, Eiji Otshubo, Daisuke Hishikawa, Satoshi Ishii, Kenji Sakimura, Masayoshi Mishina and Takao Shimizu
    • Journal Title

      Cell Metabolism

      Volume: 20 Issue: 2 Pages: 295-305

    • DOI

      10.1016/j.cmet.2014.05.019

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 新規肺胞腔内脂質結合タンパク質Sec14-like 3の生化学的機能解析2014

    • Author(s)
      菱川大介
    • Organizer
      日本生化学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of novel pulmonary surfactant lipid-related protein, Sec14-like 32014

    • Author(s)
      Daisuke Hishikawa
    • Organizer
      FASEB Science Research conferences. Lung epithelium in health & disease.
    • Place of Presentation
      Vermont Academy, Saxtons river, VT.
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 新規肺胞腔内分泌タンパク質Sec14-like 3の生化学的機能解析2014

    • Author(s)
      菱川大介
    • Organizer
      肺サーファクタント分子病態研究会
    • Place of Presentation
      札幌医科大学
    • Year and Date
      2014-06-21
    • Related Report
      2014 Research-status Report
  • [Remarks] 脂質シグナリングプロジェクトホームページ

    • URL

      http://www.ncgmlipidsp.jp

    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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