| Project/Area Number |
26860202
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | National Center for Global Health and Medicine |
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Principal Investigator |
HISHIKAWA Daisuke 国立研究開発法人国立国際医療研究センター, その他部局等, その他 (10569966)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 脂質輸送タンパク質 / 肺サーファクタント |
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| Outline of Final Research Achievements |
Although pulmonary surfactant is implicated in the various lung functions, molecular mechanisms how the amount of pulmonary surfactant in alveoli is maintained and regulated are still unclear. The aim of this study is to clarify the molecular mechanisms of pulmonary surfactant phospholipid trafficking, secretion, and recycling. To uncover these mechanisms, I focused on the Sec14L3 and Sec14L4, which is the function unknown lipid transporters, highly expressed in the lung.
In this study, I used Sec14L3 and Sec14L4 double knockout (dKO) mice to characterize the functions of these genes in vivo. There are no significant difference in the surfactant phospholipid amount and compositions between control and dKO mice, suggest that they are not critical for the basal lung function. However, I also found that the mRNA expressions of several cytokines were increased in dKO mice than that of control mice after intratracheal administration of lipopolysaccharide.
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