Investigation of a novel lipid degradation pathway focused on autophagy-lysosomal system
| Project/Area Number |
26860203
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Nihon University |
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Principal Investigator |
AIZAWA Shu 日本大学, 生物資源科学部, 助手 (10645899)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | オートファジー / リソソーム / 脂質分解 / 脂肪滴 |
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| Outline of Final Research Achievements |
The purpose of the present study was to investigate the molecular mechanisms and physiological roles of a novel type of autophagy targeting lipid molecules. RNAi-mediated silencing of a lysosomal membrane protein, which binds to lipid molecules, induced lipid droplets accumulation in cultured cells, whereas overexpression of the protein decreases fatty acid-induced lipid accumulation. We found that these effects were independent of macroautophagy. We also identified a part of lipid substrates in this pathway. Furthermore, we established knockout mice lacking the lysosomal membrane protein, and phenotypes of the mice are now investigated.
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Report
(3 results)
Research Products
(1 results)
