Functional analysis of a novel bromodomain protein in cancer cells
Project/Area Number |
26860206
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 分子標的治療 / 大腸がん / 遺伝子発現 / エピジェネティクス / ゲノム |
Outline of Final Research Achievements |
Bromodomain containing 8 (BRD8) is frequently accumulated in colorectal cancer. Although BRD8 has been considered to alter gene expression through epigenetic modification, genes regulated by BRD8 remain largely unknown. In this study, we analyzed the data of a transcriptome, ChIP-seq, and ChIA-PET, and identified a total of 49 genes that are directly regulated by BRD8. Interestingly, the list includes a gene associated with the regulation of an oncogenic signaling pathway. Consistently, knockdown of BRD8 reduced the expression of downstream gene, leading to change in the activity of the signaling pathway. Since BRD8 is a component in TRRAP/TIP60-histone acetyltransferase complex, our data may help for the development of novel strategies to treat human cancer through targeting the acetyltransferase complex.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Genetic alterations in Japanese extrahepatic biliary tract cancer.2016
Author(s)
Noguchi R, Yamaguchi K, Ikenoue T, Terakado Y, Ohta Y, Yamashita N, Kainuma O, Yokoi S, Maru Y, Nagase H, Furukawa Y
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A novel mouse model of intrahepatic cholangiocarcinoma induced by liver-specific Kras activation and Pten deletion.2016
Author(s)
Ikenoue T, Terakado Y, Nakagawa H, Hikiba Y, Fujii T, Matsubara D, Noguchi R, Zhu C, Yamamoto K, Kudo Y, Asaoka Y, Yamaguchi K, Ijichi H, Tateishi K, Fukushima N, Maeda S, Koike K, Furukawa Y
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Journal Title
Sci Rep
Volume: 印刷中
Issue: 1
Pages: 23899-23899
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Detection of APC mosaicism by next-generation sequencing in an FAP patient.2015
Author(s)
Yamaguchi K, Komura M, Yamaguchi R, Imoto S, Shimizu E, Kasuya S, Shibuya T, Hatakeyama S, Takahashi N, Ikenoue T, Hata K, Tsurita G, Shinozaki M, Suzuki Y, Sugano S, Miyano S, Furukawa Y
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Journal Title
J Hum Genet
Volume: 60(5)
Issue: 5
Pages: 227-231
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular profiles of high-grade and low-grade pseudomyxoma peritonei.2015
Author(s)
Noguchi R, Yano H, Gohda Y, Suda R, Igari T, Ohta Y, Yamashita N, Yamaguchi K, Terakado Y, Ikenoue T, Furukawa Y
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Journal Title
Cancer Med
Volume: 4(12)
Issue: 12
Pages: 1809-1816
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Disruption of Wnt/β-catenin signaling and telomeric shortening are inextricable consequences of tankyrase inhibition in human cells.2015
Author(s)
Kulak O, Chen H, Holohan B, Wu X, He H, Borek D, Otwinowski Z, Yamaguchi K, Garofalo LA, Ma Z, Wright W, Chen C, Shay JW, Zhang X, Lum L
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Journal Title
Mol Cell Biol
Volume: 35(14)
Issue: 14
Pages: 2425-2435
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Attenuated familial adenomatous polyposis with desmoids by an APC mutation2015
Author(s)
Ikenoue T, Yamaguchi K, Komura M, Imoto S, Yamaguchi R, Shimizu E, Kasuya S, Shibuya T, Hatakeyama S, Miyano S, Furukawa Y
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Journal Title
Hum Genome Variation
Volume: 2
Pages: 15011-15011
Related Report
Peer Reviewed / Open Access
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[Presentation] Efficacy of target enrichment-based sequencing for identifying genetic alterations in familial colorectal cancer.2015
Author(s)
山口貴世志, 陶弘, 倉地清隆, 前川真人, 岩泉守哉, 中村利夫, 井元清哉, 山口類, 上村光弘, 宮野悟, 椙村春彦, 古川洋一
Organizer
第74回日本癌学会学術総会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2015-10-08
Related Report
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[Presentation] Next-generation sequencing detects a mosaic APC mutation present at low allele frequencies in an FAP patient.2015
Author(s)
Kiyoshi Yamaguchi, Mitsuhiro Komura, Rui Yamaguchi, Seiya Imoto, Eigo Shimizu, Shinichi Kasuya, Tetsuo Shibuya, Seira Hatakeyama, Norihiko Takahashi, Tsuneo Ikenoue, Keisuke Hata, Giichiro Tsurita, Masaru Shinozaki, Yutaka Suzuki, Sumio Sugano, Satoru Miyano, Yoichi Furukawa
Organizer
The Clinical Genome Conference
Place of Presentation
San Francisco CA (USA)
Year and Date
2015-06-22
Related Report
Int'l Joint Research
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[Presentation] Next-generation sequencing as a potential tool in the diagnostics of APC mosaicism in FAP patient.2015
Author(s)
Kiyoshi Yamaguchi, Mitsuhiro Komura, Rui Yamaguchi, Seiya Imoto, Eigo Shimizu, Shinichi Kasuya, Tetsuo Shibuya, Seira Hatakeyama, Norihiko Takahashi, Tsuneo Ikenoue, Keisuke Hata, Giichiro Tsurita, Masaru Shinozaki, Yutaka Suzuki, Sumio Sugano, Satoru Miyano, Yoichi Furukawa
Organizer
American Association for Cancer Research 106th Annual Meeting
Place of Presentation
Philadelphia PA (USA)
Year and Date
2015-04-17
Related Report
Int'l Joint Research
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[Presentation] Search for pathogenic variants in familial colorectal cancer using a next generation sequencer2014
Author(s)
山口貴世志, 上村光弘, 清水英悟, 粕谷慎一, 山口類, 渋谷哲朗, 井元清哉, 長山聡, 高橋則彦, 池上恒雄, 菅野純夫, 鈴木穣, 宮野悟, 古川洋一
Organizer
第73回日本癌学会学術総会
Place of Presentation
横浜
Year and Date
2014-09-25 – 2014-09-27
Related Report
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