Biological activity of galectin-4 and its roles in colorectal cancer and normal tissue.

• Project/Area Number: 26860213
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kagawa University
• Principal Investigator: Ogawa Takashi 香川大学, 医学部, 助教 (80405018)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ガレクチン / 大腸がん / 消化管 / レクチン / 腸 / ツメガエル / CRISPR

Outline of Final Research Achievements

Galectin-4 is a major galectin isoform existed in the digestive tract, and it may contribute to the differentiation of epithelial cell. Recently, many immunohistochemical studies have shown that the galectin-4 is highly expressed in normal tissues, but decreased in the adenoma and the invasion front of colonic cancers. These facts suggest that the galectin-4 has specific activity, which might be involved in tumor suppression. However, the direct correlation between disappearance of galectin-4 and its molecular mechanism of tumor inhibition remain to be understood. To investigate the role of galectin-4 in tumor growth, we prepared the stable transfectants of galectin-4 in human colonic cancer cells (CRCs). Then, we found that overexpression of the galectin-4 suppresses growth of CRCs. Furthermore, knockdown of galectin-4 resulted in an increased CRCs proliferation. These results suggest that galectin-4 contributes to the suppression of colorectal cancer.

Research Products

• [Journal Article] Cooperative interactions of oligosaccharide and peptide moieties of a glycopeptide derived from IgE with galectin-9 (2016)
  • Author(s): Nakakita, S.-I., Itoh, A., Nakakita, Y., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 2 Pages: 968-979
  • DOI: 10.1074/jbc.m115.694448
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 1)Crystal structure of a Xenopus laevis skin proto-type galectin, close to but distinct from galectin-1 (2015)
  • Author(s): Nonaka Y, Ogawa T, Yoshida H, Shoji H, Nishi N, Kamitori S, Nakamura T
  • Journal Title: Glycobiology Volume: 25 Pages: 792-803
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of a Xenopus laevis skin proto-type galectin, close to but distinct from galectin-1 (2015)
  • Author(s): Nonaka Y, Ogawa T, Yoshida H, Shoji H, Nishi N, Kamitori S, Nakamura T
  • Journal Title: Glycobiology Volume: 印刷中 Issue: 7 Pages: 792-803
  • DOI: 10.1093/glycob/cwv020
  • Peer Reviewed
• [Presentation] Functional and expression analysis of Galectin-4 in Xenopus digestive tract and human colon carcinoma (2015)
  • Author(s): 小川 崇，東海林 博樹，野中 康宏, 舘野 浩章, 平林 淳, 西 望，中村 隆範
  • Organizer: BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 大腸がんにおけるガレクチン4の役割 (2015)
  • Author(s): Takashi Ogawa
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] ツメガエル消化管およびヒト大腸がん細胞におけるガレクチン4の発現及び機能解析 (2014)
  • Author(s): 小川 崇，東海林 博樹，野中 康宏, 舘野 浩章, 平林 淳, 西 望，中村 隆範
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Biological activity of galectin-4 and its roles in CRCs growth. (2014)
  • Author(s): Takashi Ogawa
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27