Establishment of mitophagy imaging method
| Project/Area Number |
26860219
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Juntendo University |
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Principal Investigator |
Inoshita Tsuyoshi 順天堂大学, 医学(系)研究科(研究院), 助教 (20601206)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | パーキンソン病 / 神経科学 / シナプス小胞 / Vps35 |
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| Outline of Final Research Achievements |
Neural function of Parkinson’s disease causative genes, PINK1 and Parkin were analyzed by using Drosophila model. Molecular genetical methods, immunostaining and live imaging showed that abnormal activity of Parkin affect accumulation of mitochondria in dopamine neurons. Neural activity were decreased by up-/down-regulation of Parkin activity and neural cell death were occurred. These results suggested that PINK1, Parkin mediated mitochondria clearance mechanisms is important for neural activity and viability.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Vps35 in cooperation with LRRK2 regulates synaptic vesicle endocytosis through the endosomal pathway in Drosophila.2017
Author(s)
1.Tsuyoshi Inoshita , Taku Arano, Yuka Hosaka, Hongrui Meng, Yujiro Umezaki, Sakiko Kosugi, Takako Morimoto, Masato Koike, Hui-Yun Chang, Yuzuru Imai, Nobutaka Hattori
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Journal Title
Human Molecular Genetics
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway.2015
Author(s)
Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, Asano T, Yoshimi K, Zhang CL, Matsumoto G, Ohtsuka T, Kageyama R, Kiyonari H, Shioi G, Nukina N, Hattori N, Takahashi R
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Journal Title
PLoS Genet.
Volume: 11
Issue: 9
Pages: e1005503-e1005503
DOI
NAID
Related Report
Peer Reviewed / Open Access
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