| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The aim of this study is to unclear the function of Smyd2 and Smyd3, both of which methylate the histone H4, in adipocyte accumulation. To compare the differentiation into adipocyte in mesenchymal stem cell and preadipocytes of Smyd2-/- and wild type mice, the expression of their adipogenic marker genes was examined. There were no significant differences in their expression between Smyd2-/- and wild type mice. The body weight of Smyd2-/-;Smyd3-/-mice elevated compared with wild type with a high-fat diet or normal chow. Smyd2-/-;Smyd3-/- mice also had higher plasma total cholesterol and free fatty acids levels than wild type mice. In further study, we will perform these experiments with other genotype mice.
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