| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Immunohistochemical analysis revealed that infiltrating macrophages were associated with more aggresive phenotype of estrogen receptor-positive breast carcinomas. Generally, macrophages are divided into M1 and M2 macrophages based on molecular markers, and most of intratumoral macrophages are M2. Coculture of MCF-7 breast caancer cells and M2-polarized THP-1 and U937 leukemic cells demonstrated that M2 macrophages enhanced migration property of breast cancer cells, while expression of estrogern recetor and estrogen-responsive genes was down-reglated in breast cancer cells.
These fndings may suggest taht breast cancer cells acquires more aggressive phenotype by interaction with M2macrophages, and lose estrogen-dependency. Therfore, it is speculated that M2 macrophages are possibly asspciated with resistance to endocrine therapy of estrogen-sensitive breast cancer and therefore M2 macrophages are considered potent therapeutic target of breast carcinomas.
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