Significance of expression loss of chromatin remodeler ARID1A in gastric cancer

• Project/Area Number: 26860232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: The University of Tokyo
• Principal Investigator: Abe Hiroyuki 東京大学, 医学部附属病院, 助教 (40708632)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 胃癌 / ARID1A / EBV / Epstein-Barr virus / クロマチンリモデリンング / 免疫組織化学 / マイクロアレイ

Outline of Final Research Achievements

Significance of ARID1A (one of the chromatin remodeling gene)　in gastric cancer was analyzed in each subtype of gastric cancer (EBV, MSI-H, and the others). Using gastric cancer cell lines, mRNA microarray revealed expression of genes related to cell proliferation or cell cycle are frequently altered by knockdown of ARID1A. Knockdown of ARID1A suppressed proliferation of gastric cancer cell lines in MTT assay. Knockdown of one gene which is overexpressed by knockdown of ARID1A resulted in decrease of infection efficiency of EBV into gastric cancer cell lines. The result indicates the importance of ARID1A in the establishment of EBV-associated gastric cancer.

Research Products

• [Journal Article] In Epstein-Barr virus-associated gastric carcinoma a high density of CD66b-positive tumor-associated neutrophils is associated with intestinal-type histology and low frequency of lymph node metastasis (2016)
  • Author(s): Abe H, Morikawa T, Saito R, Yamashita H, Seto Y, Fukayama M
  • Journal Title: Virchows Archiv Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epstein-Barr virus-associated gastric carcinoma: Use of host cell machineries and somatic gene mutations. (2015)
  • Author(s): Abe H, Kaneda A, Fukayama M.
  • Journal Title: Pathobiology Volume: 82 Issue: 5 Pages: 212-232
  • DOI: 10.1159/000434683
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 胃癌とEBウイルス (2015)
  • Author(s): 阿部浩幸、深山正久
  • Journal Title: 臨床消化器内科 Volume: 30 Pages: 769-772
• [Journal Article] Altered expression of AT-rich interactive domain 1A in hepatocellular carcinoma (2015)
  • Author(s): Abe H, Hayashi A, Kunita A, Sakamoto Y, Hasegawa K, Shibahara J, Kokudo N, Fukayama M
  • Journal Title: International Journal of Clinical and Experimental Pathology Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] CD66b-positive tumor-associated neutrophils in Epstein-Barr virus associated gastric carcinoma: a comparative study with CD8-positive cytotoxic T-lymphocytes (2016)
  • Author(s): Hiroyuki Abe, Teppei Morikawa, Ruri Saito, Hiroharu Yamashita, Yasuyuki Seto, and Masashi Fukayama
  • Organizer: Annual Meeting of the United States and Canadian Academy of Pathology
  • Place of Presentation: シアトル (アメリカ合衆国)
  • Year and Date: 2016-03-12
  • Int'l Joint Research
• [Presentation] Epstein-Barr Virus-Associated Gastric Carcinoma: the latest update (2016)
  • Author(s): Hiroyuki Abe
  • Organizer: The 10th Japan-Korea Gastrointestinal Pathology Seminar
  • Place of Presentation: 東京 (日本)
  • Year and Date: 2016-02-19
  • Int'l Joint Research
• [Presentation] EBV関連胃癌におけるmiRNA発現とexosomeを介した細胞外分泌 (2015)
  • Author(s): 阿部 浩幸、国田 朱子、日向 宗利、牛久 綾、深山 正久
  • Organizer: 第12回EBウイルス研究会
  • Place of Presentation: ビッグハート出雲 (島根県出雲市)
  • Year and Date: 2015-04-30
• [Presentation] EBV関連胃癌における好中球浸潤は組織型、深達度、及びリンパ節転移と相関する (2015)
  • Author(s): 阿部浩幸、森川鉄平、西東瑠璃、深山正久
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場 (愛知県名古屋市)
  • Year and Date: 2015-04-30
• [Presentation] EBV関連胃癌におけるARID1A発現消失はヒストンアセチル化の消失と相関する (2014)
  • Author(s): 阿部浩幸、林玲匡、国田朱子、深山正久
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26