Significance of expression loss of chromatin remodeler ARID1A in gastric cancer
| Project/Area Number |
26860232
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Abe Hiroyuki 東京大学, 医学部附属病院, 助教 (40708632)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 胃癌 / ARID1A / EBV / Epstein-Barr virus / クロマチンリモデリンング / 免疫組織化学 / マイクロアレイ |
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| Outline of Final Research Achievements |
Significance of ARID1A (one of the chromatin remodeling gene) in gastric cancer was analyzed in each subtype of gastric cancer (EBV, MSI-H, and the others). Using gastric cancer cell lines, mRNA microarray revealed expression of genes related to cell proliferation or cell cycle are frequently altered by knockdown of ARID1A. Knockdown of ARID1A suppressed proliferation of gastric cancer cell lines in MTT assay. Knockdown of one gene which is overexpressed by knockdown of ARID1A resulted in decrease of infection efficiency of EBV into gastric cancer cell lines. The result indicates the importance of ARID1A in the establishment of EBV-associated gastric cancer.
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Report
(3 results)
Research Products
(9 results)
