| Project/Area Number |
26860237
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Meguro Shiori 浜松医科大学, 医学部, 助教 (40724290)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | pericyte / 血管平滑筋細胞 / perivascular tumors / myosin 1B / perivascular tumor |
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| Outline of Final Research Achievements |
Our aim was to identify pericyte-specific markers for the analysis of formalin-fixed paraffin-embedded human tissue samples, and to characterize perivascular myoid cell neoplasms phenotypically. We compared gene expression profiles between pericytes, vascular smooth muscle cells (vSMCs), and fibroblasts, and performed human skin vasculature immunohistochemical analysis, which led to the identification of myosin 1B (MYO1B) as a novel pericyte marker. We investigated the expression levels of MYO1B and h-caldesmon (h-CD) in human skin vasculatures, and we found that the human skin vasculatures can be classified as follows: pericytes (MYO1B+h-CD-), mural cells (MYO1B+h-CD+), and vSMCs (MYO1B-h-CD+). We also examined the expression levels of MYO1B and h-CD in perivascular myoid cell neoplasms (angioleiomyomas, glomus tumors, and myopericytomas). The ability to distinguish between these cell types may allow us to understand the differentiation and origin of perivascular myoid cell neoplasms.
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