The epigenetic analysis of benign and malignant biliary tract disease with crypt isolation technique
| Project/Area Number |
26860242
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Sugai Tamotsu 岩手医科大学, 病理学講座, 教授 (20187628)
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| Co-Investigator(Renkei-kenkyūsha) |
Sasaki Akira 岩手医科大学, 外科学講座, 教授 (40275540)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 胆管癌 / 癌抑制遺伝子 / DNAメチル化 / 胆道癌 / 胆嚢癌 / メチル化 / エピジェネティクス / 癌関連遺伝子 |
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| Outline of Final Research Achievements |
We analysed the DNA methylation of its promoter region of cancer suppressor genes with cancerous glands and non-cancerous glands of bile duct cancer by crypt isolation technique. We verified the relation between the DNA methylation of cancer suppressor genes and bile duct cancer. In 31 cases of resected bile duct cancer, we picked up the fresh samples and collect cancerous glands and non-cancerous glands with crypt isolation technique. We extracted the DNA and analysed the DNA methylation of its promoter region by Bisulfite-pyrosequencing. We examined the frequencies of cancer related genes and the genome wide methylation.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Curative two-stage resection for synchronous triple cancers of the esophagus, colon, and liver: Report of a case.2015
Author(s)
Akiyama Y, Iwaya T, Konosu M, Shioi Y, Endo F, Katagiri H, Nitta H, Kimura T, Otsuka K, Koeda K, Kashiwaba M, Mizuno M, Kimura Y, Sasaki A.
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Journal Title
Int J Surg Case Rep.
Volume: 13
Pages: 1-4
DOI
Related Report
Peer Reviewed / Open Access
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